REL 2016-11-16 2016-11-18 2023-07-07 Focused Ion Beam-Scanning Electron Microscopy of mitochondrial reticulum in murine skeletal muscle Sriram Subramaniam National Cancer Institute Bethesda United States 20892 Sriram Subramaniam National Institutes of Health, NCI, Center for Cancer Research, Laboratory of Cell Biology Bethesda United States 20892 Robert Balaban National Institutes of Health, NHLBI Bethesda United States 20892 Glancy B Hartnell LM Malide D Yu ZX Combs CA Connelly PS Subramaniam S Balaban RS 1.5 10.6019/EMPIAR-10070 FIB-SEM cell Glancy B Hartnell LM Malide D Yu ZX Combs CA Connelly PS Subramaniam S Balaban RS Mitochondrial reticulum for cellular energy distribution in muscle Nature Nature 7562 523 617 620 2015 English 10.1038/nature14614 26223627
The included aligned mrc was used to provide the measured dimensions of individual mitochondria. This is a representative demonstration of the distribution of the mitochondrial reticulum through a paravascular region of murine skeletal muscle.
 aligned stack: FIB-SEM volume through skeletal muscle tissue /data reconstructed volumes MRC MRC 291 1 32 BIT FLOAT 1675 50.0 1595 50.0
MRC is of an aligned stack of tiffs that are binned by 3 in x and y. Z thickness is ~15 nm. Volume rendered components found in Figures 1 (paravascular region in murine skeletal muscle) and Figure 2 (skeletal muscle mitochondria morphologies, nucleus, and blood vessel), Glancy et al: http://www.nature.com/nature/journal/v523/n7562/pdf/nature14614.pdf are located in respective Figure folders. Software used for rendering/segmentation: ImageJ/TRAKEM2 plugin.