The pathway to GTPase activation of elongation factor SelB on the ribosome

REL 2016-11-01 2016-11-24 2016-11-24 The pathway to GTPase activation of elongation factor SelB on the ribosome 0000-0002-4609-4052 Niels Fischer Max-Planck-Institute for Biophysical Chemistry Am Fassberg 11 Göttingen Lower Saxony Germany 37073 Stark Holger Max-Planck-Institute for Biophysical Chemistry Am Fassberg 11 Goettingen Lower Saxony Germany 37073 Fischer N Neumann P Bock LV Maracci C Wang Z Paleskava A Konevega AL Schroeder GF Grubmueller H Rodnina MV Stark H 1.0 10.6019/EMPIAR-10077 EMDB EMD-4121 EMD-4122 EMD-4123 EMD-4124 EMD-4125 EMD-4126 Neumann P Bock LV Maracci C Wang Z Paleskava A Konevega AL Schroeder GF Grubmueller H Ficner R Rodnina MV Stark H The pathway to GTPase activation of elongation factor SelB on the ribosome Nature Nature 2016 English 10.1038/nature20560 27842381 Part 1 - Unprocessed cryo-EM micrographs of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complexes /data/Micrographs_part1 micrographs - single frame MRC MRC 6147 1 32 BIT FLOAT 4096 1.16 4096 1.16

Part 2 - Unprocessed cryo-EM micrographs of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complex /data/Micrographs_part2 micrographs - single frame MRC MRC 6534 1 32 BIT FLOAT 4096 1.16 4096 1.16

ribosomeSelB_particles.mrcs - CTF corrected single particle images of E. Coli 70S-SelB-GDPNP-Sec-tRNASec-fMet-tRNAfMet-SECIS mRNA complex /data picked particles - multiframe - processed MRC MRC 969526 1 32 BIT FLOAT 272 1.16 272 1.16

Particle image stack "ribosomeSelB_particles.mrcs": CTF corrected particles images (not normalized; total 969.526 particles) Star-files for particles corresponding to distinct states in "ribosomeSelB_particles.mrcs": 1. "particles_IC_state.star": particles of initial complex, E. coli ribosome 70S-fMet- tRNAfmet-SECIS mRNA complex (IC) 2. "particles_IB_state.star": particles of initial binding state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (IB) 3. "particles_CR_state.star": particles of codon reading state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (CR) 4. "particles_GA_state.star": particles of GTPase activated state of E. coli ribosome 70S-SelB-GDPNP-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (GA) 5. "particles_C_state.star": particles of classical pre-translocation state of E. coli ribosome 70S-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (C) 6. "particles_H_state.star": particles of hybrid pre-translocation state of E. coli ribosome 70S-Phe-tRNAPhe-fMet-tRNAfmet-SECIS mRNA complex (H)