REL 2017-05-17 2017-08-29 2017-08-29 Maofu Liao Department of Cell Biology, Harvard Medical School 250 Longwood Ave Boston MA United States 02115 Tom A Rapoport Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School 240 Longwood Ave Boston MA United States 1101 Maofu Liao Department of Cell Biology, Harvard Medical School 250 Longwood Ave Boston MA United States 1101 Mi WM Schoebel SS Stein AS Rapoport TAR Liao ML 708.3 10.6019/EMPIAR-10099 EMDB EMD-8639 EMD-8638 Schoebel S Mi W Stein A Ovchinnikov S Pavlovicz R DiMaio F Baker D Chambers MG Su H Li D Rapoport TA Liao M Nature Nature 2017 English 10.1038/nature23314 28682307 summed frame micrographs of Hrd1/Hrd3 complex /data/Hrd13_images micrographs - single frame MRC MRC 5361 1 32 BIT FLOAT 3838 1.35 3710 1.35

Files are named from Ucsf_stack_0001.mrc to Ucsf_stack_5361.mrc These are raw micrographs after drift correction from MotionCor2 without dose weighting.

dose-weighted summed frame micrographs of Hrd1/Hrd3 complex /data/Hrd13_images_DW micrographs - single frame MRC MRC 5361 1 32 BIT FLOAT 3838 1.35 3710 1.35

Files are named from Ucsf_stack_0001_DW.mrc to Ucsf_stack_5361_DW.mrc These are summed micrographs after drift correction from MotionCor2 with dose-weighting

picked particles of Hrd1/Hrd3 complex /data/Hrd13_particles picked particles - multiframe - processed MRC MRC 1015288 1 32 BIT FLOAT 192 1.35 192 1.35

particles are in three files Hrd13_July25-26_fixs5_i_n1.mrc Hrd13_July27-28_fixs5_i_n1.mrc Hrd13_July29-31_fixs5_i_n1.mrc total particle number from above three files is 1015288. corresponding star files are available as well Under folder Hrd13_svco-files_bin3_1015288 are coordinate files of picked particles, and files are SPIDER format. The coordinates are for images binned by 3, so if the users want to box particles from raw images, they need to multiple 3 for all coordinates to get the right value.