REL 2017-12-05 2017-12-15 2022-07-14 0000-0002-0624-5020 Bridget Carragher New York Structural Biology Center 89 Convent Avenue New York NY United States 10027 0000-0002-0624-5020 Bridget Carragher New York Structural Biology Center 89 Convent Avenue New York NY United States 10027 Noble AJ Dandey VP Wei H Brasch J Chase J Acharya P Tan YZ Zhang Z Kim LY Scapin G Rapp M Eng ET Rice WJ Cheng A Negro CJ Shapiro L Kwong PD Jeruzalmi D des Georges A Potter CS Carragher B 998.3 10.6019/EMPIAR-10130 EMDB EMD-7138 Noble AJ Dandey VP Wei H Brasch J Chase J Acharya P Tan YZ Zhang Z Kim LY Scapin G Rapp M Eng ET Rice WJ Cheng A Negro CJ Shapiro L Kwong PD Jeruzalmi D des Georges A Potter CS Carragher B eLife Elife 7 2018 English 10.7554/elife.34257 29809143 Super-resolution K2 tilt-series frames /data/frames micrographs - multiframe MRCS MRC 60 5 UNSIGNED 16 BIT INTEGER 7420 0.5595 7676 0.5595

- 1 mg/ml rabbit muscle aldolase on a gold Spotiton nanowire grid, - Collected bi-directionally from [-12:45] then [-12:-45] with 3 degree tilt increment, - Collected ~50 nm away from the edge of 1-2 micron diameter holes because farther out there are no particles, - Ice is about 15-25 nm thin, - Collected with a random defocus range between 1.2 and 2.2 microns, - Collected at 105kx mag = 1.119 angstrom pixelsize, 0.5595 angstrom pixelsize at super-res (calibrated: 0.530245), - 15-20 eV slit at ZLP (I think most was 15 eV, I don't remember), re-aligned automatically every 1.25 hours, - ~8 e-/px/s dose rate on the camera, - 1.54 e-/A^2 for tilt angles up to 24 degrees, 1.84 e-/A^2 for 27 to 39 degrees, 2.15 e-/A^2 for 42 and 45 degrees, - 100 ms frames, - 5-7 frames per tilt image, depending on tilt (cosine increase in dose), - ~< 1 angstrom per-image drift at all tilt angles, - tiltseries0003 and tiltseries0004 were collected without the objective aperture in. - Gain and dark references included.

Whole-frame aligned tilt images along with all other magnification images from the collection /data/leginon micrographs - single frame MRC MRC 60 5 32 BIT FLOAT 7420 0.5595 7676 0.5595

Whole-frame aligned tilt images using Motioncor2. Calibrated pixelsize: 0.530245

Appion-Protomo tilt-series alignments /data/protomo_alignments tilt series MRC MRC 30 5 32 BIT FLOAT 7420 0.5595 7676 0.5595

Includes a full tilt-series alignment run for 30 of the ~50 good tilt-series. To view properly, navigate to the protomo_alignments folder in Appion-Protomo. Calibrated pixelsize: 0.530245