REL
2018-01-18
2018-03-20
2018-03-20
Cryo-electron tomography of the yeast NPC
0000-0002-3059-3121
Christopher
Winslow
Akey
Department of Physiology and Biophysics, Boston University School of Medicine
700 Albany Street
Boston
Massachusetts
United States
02118
0000-0002-3059-3121
Christopher
Winslow
Akey
Department of Physiology and Biophysics, Boston University School of Medicine
700 Albany Street
Boston
Massachusetts
United States
02118
Steven
J
Ludtke
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
1 Baylor Plaza
Houston
Texas
United States
77030
Michael
P
Rout
Laboratory of Cellular and Structural Biology, Rockefeller University
1230 York Avenue
New York
New York
United States
10065
Nudelman I
Fernandez-Martinez J
Rout MP
Ludtke SJ
Akey CW
127.1
10.6019/EMPIAR-10155
EMDB
EMD-7321
Kim SJ
Fernandez-Martinez J
Nudelman I
Shi Y
Zhang W
Raveh B
Herricks T
Slaughter BD
Hogan J
Upla P
Chemmama IE
Pellarin R
Echeverria I
Shivaraju M
Chaudhury AS
Wang J
Williams R
Unruh JR
Greenberg CH
Jacobs EY
Yu Z
Jason de la Cruz M
Mironska R
Stokes DL
Aitchison JD
Jarrold MF
Gerton JL
Ludtke SJ
Akey CW
Chait BT
Sali A
Rout MP
Hogan JA
de la Cruz MJ
Integrative structure and functional anatomy of a nuclear pore complex
Nature
Nature
United Kingdom
2018
English
10.1038/nature26003
29539637
An integrative structure with subnanometer precision was determined for the entire 552-protein yeast NPC by satisfying diverse data, including a 3D cryo-EM map obtained by averaging sub-volumes from tomograms, stoichiometry measurements, and chemical cross-links.
Unaligned K2 DED image tilt series of yeast NPC and associated files
/data/tiltseries
micrographs - single frame
MRC
MRC
120
1
UNSIGNED 16 BIT INTEGER
3710
5.6
3710
5.6
Data collected with UCSF Tomo and best fit value for pixel size is 5.3 Angstroms. Variable number of tilts in the tilt series, due to different sampling and some higher tilted images with bad drift were removed. Additional files are provided , adoc parameter files used
in IMOD Batchtomo, list files with nominal and fitted tilt angles and
fitted defocus values. Coordinate files for particles selected with
e2spt_boxer.py are also given, a box size of 300 was used for
final processing with unbinned data.