REL
2018-03-27
2018-05-30
2018-08-30
Structure of a pre-catalytic spliceosome
0000-0002-0462-6540
Sjors
HW
Scheres
MRC laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0002-0462-6540
Sjors
HW
Scheres
MRC laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Plaschka C
Lin PC
Nagai K
Nakane T
Kimanius D
Lindahl E
Scheres SHW
126.5
10.6019/EMPIAR-10180
EMDB
EMD-3682
EMD-3683
EMD-3684
EMD-3685
EMD-3686
EMD-3687
EMD-3688
Plaschka C
Lin PC
Nagai K
Structure of a pre-catalytic spliceosome
Nature
Nature
7660
546
617
621
2017
English
10.1038/nature22799
28530653
Nakane T
Kimanius D
Lindahl E
Scheres SH
Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION
eLife
Elife
United Kingdom
7
2018
English
10.7554/eLife.36861
29856314
Polished parcitles
/data
picked particles - single frame - processed
MRCS
MRCS
327490
1
32 BIT FLOAT
320
1.699
320
1.699
Shiny particles after particle polishing used for the Nature paper were further down-sampled to 1.699 Å/px and windowed to 320 px. These particles were used in the Multibody paper.
The particle STAR file after consensus refinement, masks and initial references for four bodies and a body STAR file are also included. Readers can reproduce the multibody refinement in the paper using these inputs.