Separating distinct macromolecular assemblies from cryo-EM images

REL 2019-04-16 2020-07-14 2020-07-14 Separating distinct macromolecular assemblies from cryo-EM images 0000-0002-6198-1194 David W. Taylor Department of Molecular Biosciences, University of Texas at Austin 100 E. 24th St. Aust TX United States 78712 0000-0002-6198-1194 David W. Taylor Department of Molecular Biosciences, University of Texas at Austin 100 E. 24th St. Aust TX United States 78712 Edward M. Marcotte Department of Molecular Biosciences, University of Texas at Austin 100 E. 24th St. Austin TX United States 78712 Verbeke EJ Zhou Y Horton AP Mallam AL Taylor DW Marcotte EM 128.5 10.6019/EMPIAR-10268 EMDB EMD-20109 EMD-20110 EMD-20111 EMD-20112 Verbeke EJ Zhou Y Horton AP Mallam AL Taylor DW Marcotte EM Separating distinct structures of multiple macromolecular assemblies from cryo-EM projections Journal of structural biology J. Struct. Biol. 1 209 2019 English 10.1016/j.jsb.2019.107416 31726096 Drift-corrected and dose-weighted average micographs of a mixture containing 40S, 60S, 80S and apoferritin /data micrographs - single frame MRC MRC 2423 1 32 BIT FLOAT 3710 1.1 3838 1.1

These micrographs are the average after alignment and dose-weighting of 20 raw movie frames (accumulating 40 electron per squared Angstrom in a 6 second exposure).