Micrographs of E. coli 70S ribosomes collected at 100 keV using a hybrid pixel direct electron detector

REL 2019-08-27 2020-03-23 2019-08-28 Micrographs of E. coli 70S ribosomes collected at 100 keV using a hybrid pixel direct electron detector 0000-0002-4442-744X Christopher John Russo MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0002-4442-744X Christopher John Russo MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH Naydenova K McMullan G Peet MJ Lee Y Edwards PC Chen S Leahy E Scotcher S Henderson R Russo CJ 4.0 10.6019/EMPIAR-10316 EMDB EMD-10265 Naydenova K McMullan G Peet MJ Lee Y Edwards PC Chen S Leahy E Scotcher S Henderson R Russo CJ CryoEM at 100 keV: a demonstration and prospects IUCrJ IUCrJ Pt 6 6 1086 1098 2019 English 10.1107/S2052252519012612 31709064

Raw movie frames (32, no gain correction, no defect correction) accumulating 16.6 electrons (100 keV) per squared Angstrom in a 2 second exposure.

Raw multi-frame micrographs of E. coli 70S ribosomes recorded at 100 keV /data micrographs - multiframe MRCS MRCS 127 32 1 32 SIGNED 16 BIT INTEGER 1030 2.74 514 2.74

Unaligned movie frames (32) accumulating 16.6 electrons per squared Angstrom in a 2 second exposure.