REL
2020-01-16
2020-08-25
2020-08-25
FAK structure from single particle analysis of 2D crystals
0000-0003-4247-4303
Ricardo
Righetto
Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel
Mattenstrasse 26
Basel
Switzerland
4058
0000-0002-6133-6486
Daniel
Lietha
Structural and Chemical Biology, Biological Research Center (CIB-CSIC)
Madrid
Spain
28040
0000-0002-1185-4592
Henning
Stahlberg
Center for Cellular Imaging and NanoAnalytics, Biozentrum, University of Basel
Mattenstrasse 26
Basel
Switzerland
4058
Acebron I
Righetto RD
Biyani N
Chami M
Boskovic J
Stahlberg H
Lietha D
1.8
10.6019/EMPIAR-10347
EMDB
EMD-10615
EMD-10616
Acebrón I
Righetto RD
Schoenherr C
de Buhr S
Redondo P
Culley J
Rodríguez CF
Daday C
Biyani N
Llorca O
Byron A
Chami M
Gräter F
Boskovic J
Frame MC
Stahlberg H
Lietha D
Structural basis of Focal Adhesion Kinase activation on lipid membranes
The EMBO journal
EMBO J.
2020
English
10.15252/embj.2020104743
32779739
Dose-weighted aligned movie averages of the FAK AMP-PNP dataset
/data/amp-pnp/micrographs/alignaver
micrographs - single frame
MRC
MRC
2513
1
32 BIT FLOAT
3838
1.058
3710
1.058
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
Micrographs have been downsampled by a factor of two in relation to the super-resolution movies, i.e. they are at the physical pixel size.
More details in the data/README file.
data/amp-pnp/micrographs/alignaver/*.mrc
data/amp-pnp/micrographs/coordinates/*.box
data/amp-pnp/particles/
Unaligned movies of the FAK AMP-PNP dataset
/data/amp-pnp/micrographs/raw
micrographs - multiframe
TIFF
TIFF
2513
40
UNSIGNED BYTE
7676
0.529
7420
0.529
Movies have been acquired in super-resolution mode of the K2 detector.
The gain reference and defects list file are stored under data/amp-pnp/micrographs/gainref/.
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
More details in the data/README file.
data/amp-pnp/micrographs/alignaver/*.mrc
data/amp-pnp/micrographs/coordinates/*.box
data/amp-pnp/particles/
Stack of particles extracted from the FAK AMP-PNP dataset
/data/amp-pnp/particles
picked particles - single frame - unprocessed
MRCS
MRCS
2666154
1
32 BIT FLOAT
200
2.116
200
2.116
Particle boxes have been downsampled by a factor of two in relation to the aligned micrographs.
The initial orientations and defocii values are determined from the 2D crystal processing are available in the particles.star file.
More details in the data/README file.
data/amp-pnp/micrographs/alignaver/*.mrc
data/amp-pnp/micrographs/coordinates/*.box
data/amp-pnp/particles
Dose-weighted aligned movie averages of the FAK APO Polara dataset
/data/apo/micrographs/polara/alignaver
micrographs - single frame
MRC
MRC
88
1
32 BIT FLOAT
3808
1.7
3808
1.7
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
More details in the data/README file.
data/apo/micrographs/polara/alignaver/*.mrc
data/apo/micrographs/polara/coordinates/*.box
data/apo/particles/
Dose-weighted aligned movie averages of the FAK APO Titan dataset
/data/apo/micrographs/titan/alignaver
micrographs - single frame
MRC
MRC
509
1
32 BIT FLOAT
3808
1.058
3808
1.058
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
More details in the data/README file.
data/apo/micrographs/titan/alignaver/*.mrc
data/apo/micrographs/titan/coordinates/*.box
data/apo/particles/
Unaligned movies of the FAK APO Titan dataset
/data/apo/micrographs/titan/raw
micrographs - multiframe
TIFF
TIFF
509
80
UNSIGNED BYTE
7676
0.529
7420
0.529
Movies have been acquired in super-resolution mode of the K2 detector.
The gain reference and defects list file are stored under data/apo/micrographs/titan/gainref/.
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
More details in the data/README file.
data/apo/micrographs/titan/alignaver/*.mrc
data/apo/micrographs/titan/coordinates/*.box
data/apo/particles/
Stack of particles extracted from the FAK APO Titan and Polara datasets
/data/apo/particles
picked particles - single frame - unprocessed
MRCS
MRCS
361796
1
32 BIT FLOAT
238
1.7
238
1.7
Particle boxes extracted from the Titan dataset have been downsampled to approximately match the pixel size of the Polara dataset. The stack contains all particles extracted from the FAK APO Titan and Polara datasets merged.
The initial orientations and defocii values are determined from the 2D crystal processing are available in the particles_preprocessed.star file.
More details in the data/README file.
data/apo/micrographs/titan/alignaver/*.mrc
data/apo/micrographs/titan/coordinates/*.box
data/apo/particles/