REL
2020-03-27
2020-05-26
2020-04-21
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Wild-type
0000-0001-6656-6320
Yong Zi
Tan
Department of Physiology and Cellular Biophysics, Columbia University
1150 Saint Nicolas Ave
New York
New York
United States
10032
0000-0003-3293-2200
Filippo
Mancia
Department of Physiology and Cellular Biophysics, Columbia University
1150 Saint Nicolas Ave
New York
New York
United States
10032
0000-0002-0624-5020
Bridget
Carragher
New York Structural Biology Center/Columbia University
89 Convent Ave
New York
New York
United States
10027
0000-0003-2394-0831
Clinton
S
Potter
New York Structural Biology Center/Columbia University
89 Convent Ave
New York
New York
United States
10027
Tan YZ
Zhang L
Rodrigues J
Zheng RB
Giacometti SI
Rosário AL
Kloss B
Dandey VP
Wei H
Brunton R
Raczkowski AM
Athayde D
Catalão MJ
Pimentel M
Clarke OB
Lowary TL
Archer M
Niederweis M
Potter CS
Carragher B
Mancia F
2.7
10.6019/EMPIAR-10399
EMDB
EMD-21580
Tan YZ
Zhang L
Rodrigues J
Zheng RB
Giacometti SI
Rosário AL
Kloss B
Dandey VP
Wei H
Brunton R
Raczkowski AM
Athayde D
Catalão MJ
Pimentel M
Clarke OB
Lowary TL
Archer M
Niederweis M
Potter CS
Carragher B
Mancia F
Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria
Molecular cell
Mol. Cell
2020
English
10.1016/j.molcel.2020.04.014
32386575
Unaligned and compressed multi-frame movies
/data/01_Raw_Frames
micrographs - multiframe
MRCS
MRCS
7354
90
1
90
UNSIGNED 16 BIT INTEGER
3838
1.0605
3710
1.0605
Data collection was performed using a dose of ~95.72 e-/Å2 across 90 frames (150 msec per frame) at a dose rate of ~8.2 e-/pix/sec, using a set defocus range of -1.0 μm to -2.0 μm. Raw frames were compressed using pbzip2. Gain reference image to use are in each of the four folders under references/*_3838x3710_norm_0_mod.mrc.
data/02_Aligned_Micrographs/*mrc
data/03_Final_Stack/Final_AftD-WT.star
data/03_Final_Stack
Aligned and dose-weighted micrographs
/data/02_Aligned_Micrographs
micrographs - single frame
MRC
MRC
7274
1
32 BIT FLOAT
3838
1.0605
3710
1.0605
Movie frames were aligned using MotionCor2 with 5 by 5 patches and B-factor of 100 through the Appion software package.
data/02_Aligned_Micrographs/*mrc
data/03_Final_Stack/Final_AftD-WT.star
data/03_Final_Stack
Final Particle Stacks with Refined Euler Angles and Shifts (Overall and Focused Refined on CBM3)
/data/03_Final_Stack
picked particles - single frame - processed
MRCS
MRCS
2
1
32 BIT FLOAT
288
1.0605
288
1.0605
Final particle stacks, with Relion .star file containing the refined Euler angles and shifts. Final_AftD-WT.star has the refined Euler angles and shifts for the entire dataset, while FocussedClassified_CBM3_AftD-WT.star has the refined Euler angles and shifts for just the subset of particles after CBM3 focused classification.
data/02_Aligned_Micrographs/*mrc
data/03_Final_Stack/Final_AftD-WT.star
data/03_Final_Stack