Motion-corrected micrographs and extracted particle images of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with tigecycline

REL 2020-05-07 2020-11-24 2021-04-23 Motion-corrected micrographs and extracted particle images of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with tigecycline 0000-0001-5404-8251 David Nicholson Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds Leeds West Yorkshire United Kingdom LS2 9JT 0000-0002-1467-3674 Thomas A Edwards Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds Leeds West Yorkshire United Kingdom LS2 9JT 0000-0003-3222-5493 Alex J O'Neill Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds Leeds West Yorkshire United Kingdom LS2 9JT 0000-0002-3640-5275 Neil A Ranson Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds Leeds West Yorkshire United Kingdom LS2 9JT Nicholson D Edwards TA O'Neill AJ Ranson NA 538.5 10.6019/EMPIAR-10407 EMDB EMD-10898 EMD-10914 EMD-10915 Nicholson D Edwards TA O'Neill AJ Ranson NA Structure of the 70S Ribosome from the Human Pathogen Acinetobacter baumannii in Complex with Clinically Relevant Antibiotics Structure (London, England : 1993) Structure 10 28 1087 1100.e3 2020 English 10.1016/j.str.2020.08.004 32857965

Drift-corrected and dose-corrected averages of movies collected at an accumulated dose of 62 e-/A^2 across 43 frames over a 1.1 s exposure, recorded by a ThermoFisher Falcon 3EC detector.

Motion-corrected micrographs of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with tigecycline /data/acinetobacter_baumannii_ribosome_tigecycline/micrographs micrographs - single frame MRC MRC 6228 1 32 BIT FLOAT 4096 1.065 4096 1.065

Drift-corrected and dose-corrected averages of movies collected at an accumulated dose of 62 e-/A^2 across 43 frames over a 1.1 s exposure, recorded by a ThermoFisher Falcon 3EC detector. Motion correction was performed using MotionCor2 within RELION 3.0, across frames 3-43, applying a B-factor of 150, and using 5 patches in both the X and Y directions. Coordinates corresponding to extracted particle images are in the particles.star file.

data/acinetobacter_baumannii_ribosome_tigecycline/micrographs data/acinetobacter_baumannii_ribosome_tigecycline/particles/particles.star data/acinetobacter_baumannii_ribosome_tigecycline/particles Extracted particle images of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with tigecycline /data/acinetobacter_baumannii_ribosome_tigecycline/particles picked particles - multiframe - processed MRCS MRCS 6197 1 32 BIT FLOAT 416 1.065 416 1.065

Particles were extracted from motion-corrected, CTF-estimated micrographs into 416 square pixel boxes with re-centring of refined coordinates. Images of particles extracted from each micrograph are stacked into separate mrcs files. Coordinates and further metadata corresponding to extracted particle images are in the particle.star file.

data/acinetobacter_baumannii_ribosome_tigecycline/micrographs data/acinetobacter_baumannii_ribosome_tigecycline/particles/particles.star data/acinetobacter_baumannii_ribosome_tigecycline/particles