REL
2020-05-29
2020-06-16
2021-01-22
Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis
0000-0003-0863-7826
Jeffrey
B
Bonanno
Anbert Einstein College of Medicine
Bronx
NY
United States
10461
0000-0002-4863-0015
Jonathan
Lai
Albert Einstein College of Medicine
Bronx
NY
United States
10461
0000-0002-8014-7269
Edward
Eng
New York Structural Biology Center
New York
NY
United States
10027
0000-0003-2591-5234
Steven
C
Almo
Albert Einstein College of Medicine
Bronx
NY
United States
10461
Herrera NG
Morano NC
Celikgil A
Georgiev GI
Malonis R
Lee JH
Tong K
Vergnolle O
Massimi A
Yen LY
Noble AJ
Kopylov M
Bonanno JB
Garrett-Thomson SC
Hayes DB
Bortz R
Wirchnianski A
Florez C
Laudermilch E
Haslwanter D
Fels J
Dieterle M
Jangra R
Barnhill J
Mengotto A
Kimmel D
Daily J
Pirofski L
Chandran K
Brenowitz M
Garforth S
Eng E
Lai JR
Almo SC
484.3
10.6019/EMPIAR-10433
EMDB
EMD-22078
Herrera NG
Morano NC
Celikgil A
Georgiev GI
Malonis R
Lee JH
Tong K
Vergnolle O
Massimi A
Yen LY
Noble AJ
Kopylov M
Bonanno JB
Garrett-Thomson SC
Hayes DB
Bortz R
Wirchnianski A
Florez C
Laudermilch E
Haslwanter D
Fels J
Dieterle M
Jangra R
Barnhill J
Mengotto A
Kimmel D
Daily J
Pirofski L
Chandran K
Brenowitz M
Garforth S
Eng E
Lai JR
Almo SC
Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis
ACS omega
ACS Omega
2020
English
10.1021/acsomega.0c03512
Unaligned multi frame micrographs of SARS CoV-2 SPIKE protein produced in CHO cells
/data
micrographs - multiframe
TIFF
TIFF
1280
50
UNSIGNED BYTE
5760
variable
4092
variable
Topaz picking model exported from CryoSPARC. To use the model, you can:
1) Import it back into CryoSPARC by following these instructions: https://discuss.cryosparc.com/t/import-the-topaz-covid-19-spike-picking-model-to-use-in-your-projects/4708
2) Use the Topaz .sav file (located in P257_J75_topaz_model/J75/models/model_epoch11.sav) to pick with the Topaz standalone GUI or any other software that integrates Topaz (e.g. Relion or Scipion).
Processed data - aligned frame sum with dose-weighting
/data
micrographs - single frame
MRC
MRC
1280
1
32 BIT FLOAT
5760
1.058
4092
1.058
The high mag images (-a-DW.mrc) are 66.49 e-/A2 dose at 1.058Å/px with a dimension of 5760x4092px
Topaz picking model exported from CryoSPARC. To use the model, you can:
1) Import it back into CryoSPARC by following these instructions: https://discuss.cryosparc.com/t/import-the-topaz-covid-19-spike-picking-model-to-use-in-your-projects/4708
2) Use the Topaz .sav file (located in P257_J75_topaz_model/J75/models/model_epoch11.sav) to pick with the Topaz standalone GUI or any other software that integrates Topaz (e.g. Relion or Scipion).