REL 2020-06-11 2020-08-18 2020-08-18 0000-0002-4609-4052 Niels Fischer Max-Planck-Institute for Biophysical Chemistry Am Fassberg 11 Göttingen Lower Saxony Germany 37073 0000-0003-0105-3879 Marina V. Rodnina Max-Planck-Institute for Biophysical Chemistry Am Fassberg 11 Göttingen Lower Saxony Germany 37073 0000-0002-4609-4052 Niels Fischer Max-Planck-Institute for Biophysical Chemistry Am Fassberg 11 Göttingen Lower Saxony Germany 37073 0000-0003-3816-3828 Daniel N. Wilson University of Hamburg Martin-Luther-King-Platz 6 Hamburg Hamburg Germany 20146 Chan K-H Petrychenko V Mueller C Maracci C Holtkamp W Wilson DN Fischer N Rodnina MV 788.2 10.6019/EMPIAR-10443 EMDB EMD-10905 EMD-10906 EMD-10907 EMD-10908 Chan K-H Petrychenko V Mueller C Maracci C Holtkamp W Wilson DN Fischer N Rodnina MV Larochelle SL Nature Communications Nat. Commun. United Kingdom 1 11 2020 English 10.1038/s41467-020-17853-7 27906160 Motion-corrected, dose-weighted micrographs & polished particles of P+9 E. coli ribosome-ArfB complex /data/P+9 E. Coli ribosome-ArfB complex micrographs - single frame MRC MRC 4750 1 32 BIT FLOAT 4096 1.16 4096 1.16

Note: Micrographs were obtained from movie-stacks of 40 frames à 1.25 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion, "shiny" particles were obtained by Baysian polishing in Relion 3.0 - Micrographs .star-file: "micrographs_ctf.star" - Particles .star-file for all particles: "particles_all.star" - Particles .star-file for particles of the P+9 stalled ribosome complex (EMD-10905): "particles_P+9_stalled_complex.star" - Particles .star-file for particles of P+9 ArfB-ribosome complex in the post-hydrolysis state (EMD-10906): "particles_P+9 post-hydrolysis state.star" - Particles .star-file for particles of P+9 ArfB-ribosome complex with P/E hybrid tRNA in the post-hydrolysis state (EMD-10907): "particles_P+9 post-hydrolysis state with PE hybrid tRNA.star"

data/P+9 E. Coli ribosome-ArfB complex/Micrographs/FoilHole_*_Data_*_Fractions.mrc data/P+9 E. Coli ribosome-ArfB complex/particles_*.star data/P+9 E. Coli ribosome-ArfB complex/Particles Motion-corrected, dose-weighted micrographs & extracted particles of P+0 E. coli ribosome-ArfB complex /data/P+0 E. Coli ribosome-ArfB complex micrographs - single frame MRC MRC 3633 1 32 BIT FLOAT 4096 1.072 4096 1.072

Note: Micrographs were obtained from movie-stacks of 10 frames à 2.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion - Micrographs .star-file: "micrographs_ctf.star" - Particles .star-file for particles of P+0 ArfB-ribosome complex in the post-hydrolysis state (EMD-10908): "particles_P+0 post-hydrolysis state.star"

data/P+0 E. Coli ribosome-ArfB complex/Micrographs/sum_mc2_0to9_*.mrc data/P+0 E. Coli ribosome-ArfB complex/particles_P+0 post-hydrolysis state.star data/P+0 E. Coli ribosome-ArfB complex/Particles