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        <keyDates>
            <depositionDate>2020-06-03</depositionDate>
            <releaseDate>2020-10-16</releaseDate>
            <updateDate>2020-10-16</updateDate>
        </keyDates>
        <title>Tilt-series from cryo-lamellae of WT S. cerevisiae cells</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-7397-1321</authorORCID>
            <firstName>Martin</firstName>
            <lastName>Beck</lastName>
            <organization type="academic">Department of Molecular Sociology, Max Planck Institute of  Biophysics</organization>
            <street>Max-von-Laue Str. 3</street>
            <townOrCity>Frankfurt am Main</townOrCity>
            <country>Germany</country>
            <postOrZipCode>60438</postOrZipCode>
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            <firstName>Matteo</firstName>
            <lastName>Allegretti</lastName>
            <organization type="academic">EMBL European molecular biology laboratory</organization>
            <street>Meyerhofstrasse</street>
            <townOrCity>Heidelberg</townOrCity>
            <stateOrProvince>Baden-Württemberg</stateOrProvince>
            <country>Germany</country>
            <postOrZipCode>69117</postOrZipCode>
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        <authorsList>
            <author authorORCID="0000-0002-9542-7482">Allegretti M</author>
            <author>Zimmerli CE</author>
            <author authorORCID="0000-0003-4768-3091">Rantos V</author>
            <author authorORCID="0000-0002-6559-7261">Wilfling F</author>
            <author>Ronchi P</author>
            <author>Fung HKH</author>
            <author authorORCID="0000-0002-8925-7421">Lee CW</author>
            <author authorORCID="0000-0001-6229-2692">Hagen W</author>
            <author authorORCID="0000-0002-5457-4478">Turoňová B</author>
            <author>Karius K</author>
            <author authorORCID="0000-0002-4354-891X">Börmel M</author>
            <author>Zhang X</author>
            <author authorORCID="0000-0003-2176-8337">Müller CW</author>
            <author>Schwab Y</author>
            <author authorORCID="0000-0001-6968-041X">Mahamid J</author>
            <author authorORCID="0000-0003-2180-5054">Pfander B</author>
            <author authorORCID="0000-0002-3641-0322">Kosinski J</author>
            <author authorORCID="0000-0002-7397-1321">Beck M</author>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-10198</emdbEntry>
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        <citationList>
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                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-9542-7482" order="1">Allegretti M</author>
                    <author order="2">Zimmerli CE</author>
                    <author authorORCID="0000-0003-4768-3091" order="3">Rantos V</author>
                    <author authorORCID="0000-0002-6559-7261" order="4">Wilfling F</author>
                    <author order="5">Ronchi P</author>
                    <author order="6">Fung HKH</author>
                    <author authorORCID="0000-0002-8925-7421" order="7">Lee CW</author>
                    <author authorORCID="0000-0001-6229-2692" order="8">Hagen W</author>
                    <author authorORCID="0000-0002-5457-4478" order="9">Turoňová B</author>
                    <author order="10">Karius K</author>
                    <author authorORCID="0000-0002-4354-891X" order="11">Börmel M</author>
                    <author order="12">Zhang X</author>
                    <author authorORCID="0000-0003-2176-8337" order="13">Müller CW</author>
                    <author order="14">Schwab Y</author>
                    <author authorORCID="0000-0001-6968-041X" order="15">Mahamid J</author>
                    <author authorORCID="0000-0003-2180-5054" order="16">Pfander B</author>
                    <author authorORCID="0000-0002-3641-0322" order="17">Kosinski J</author>
                    <author authorORCID="0000-0002-7397-1321" order="18">Beck M</author>
                    <title>In-cell architecture of the nuclear pore and snapshots of its turnover</title>
                    <journal>Nature</journal>
                    <journalAbbreviation>Nature</journalAbbreviation>
                    <country>Germany</country>
                    <year>2020</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41586-020-2670-5</externalReferences>
                    <externalReferences type="pubmed">32879490</externalReferences>
                    <details>Each tilt is the aligned average of ~15 movie frames performed during automatic SerialEM acquisition.</details>
                </journalCitation>
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    </crossReferences>
    <imageSet>
        <name>Raw tilt series from S. cerevisiae WT cells containing nuclear pore complexes (each tilt is an aligned average of ~15 frames by serial em)</name>
        <directory>/data/raw_tiltseries_mrc</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>177</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3708</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>3838</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details>The number of tilts varies between tilt-series</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Tilt series of WT cerevisiae cells after cleaning bad tilts and dose filtering</name>
        <directory>/data/tiltseries_st_after_cleaning_and_filtering_the_tilts</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>177</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
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            <imageWidth>3708</imageWidth>
            <pixelWidth>variable</pixelWidth>
            <imageHeight>3708</imageHeight>
            <pixelHeight>variable</pixelHeight>
        </dimensions>
        <details>The number of tilts varies between tilt-series</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
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