REL 2020-07-15 2020-09-11 2022-02-25 0000-0003-4295-1229 Tara Charlie Marcink Department of Pediatrics, Columbia University Medical Center New York New York United States 10032 0000-0002-1796-8320 Anne Moscona Department of Pediatrics, Columbia University Medical Center New York New York United States 10032 0000-0003-3866-9220 Matteo Porotto Department of Pediatrics, Columbia University Medical Center New York New York United States 10032 0000-0002-9704-3781 Amedee des Georges Department of Chemistry & Biochemistry, The City College of New York New York New York United States 10031 Marcink TC Wang T des Georges A Porotto M Moscona A 11.6 10.6019/EMPIAR-10476 EMDB EMD-22334 Marcink TC Wang T des Georges A Porotto M Moscona A PLoS pathogens PLoS Pathog 9 16 2020 English 10.1371/journal.ppat.1008883 32956394 Human parainfluenza virus fusion complex glycoproteins imaged in action on authentic viral surfaces: Tomograms of HPIV3 prior to receptor engagement /data tilt series MRC MRC 2 1 32 BIT FLOAT 1919 variable 1855 variable

Tilt series were collected at 3 degree steps from -51 to 51 degrees with a 3.5 micron defocus using SerialEM with a total dose of 120 e-/Å2. Micrographs were aligned by MotionCorr2 and tomograms were aligned and reconstructed using Appion-Protomo.