REL 2020-05-24 2020-11-13 2020-11-13 0000-0002-0462-6540 Sjors Scheres MRC LMB Francis Crick Avenue Cambridge United Kingdom CB2 0QH Sjors Scheres MRC LMB Cambridge United Kingdom CB2 0QH Nakane T Kotecha A Sente A McMullan G Masiulis S Brown PMGE Grigoras IT Malinauskaite L Malinauskas T Miehling J Uchański T Yu L Karia D Pechnikova EV de Jong E Keizer J Bischoff M McCormack J Tiemeijer P Hardwick SW Chirgadze DY Murshudov G Aricescu AR Scheres SHW 13.0 10.6019/EMPIAR-10500 EMDB EMD-11657 Nakane T Kotecha A Sente A McMullan G Masiulis S Brown PMGE Grigoras IT Malinauskaite L Malinauskas T Miehling J Uchański T Yu L Karia D Pechnikova EV de Jong E Keizer J Bischoff M McCormack J Tiemeijer P Hardwick SW Chirgadze DY Murshudov G Aricescu AR Scheres SHW Nature Nature 2020 English 10.1038/s41586-020-2829-0 33087931

This deposition contains raw movies of GABAAR beta3 homopentamer. The apoferritin dataset described in the same paper is available in EMPIAR-10424. In addition to raw movies, polished particles are deposited in the PolishedParticles directory. Five out of eight datasets, CFEG-5eV-noOA, CFEG-5eV-withOA, XFEG-10eV,XFEG-5eV and XFEG-3eV were merged to yield the final map. The merged and re-refined STAR file is also available (merged.star).

CFEG, Falcon4, with 5 eV filter /data/CFEG-5eV-withOA micrographs - multiframe EER EER 5573 1113 1 1113 BIT 4096 0.727 4096 0.727

Total dose: 40 e/A2 on sample Please use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.

CFEG, Falcon4, with 5 eV filter, without objective aperture /data/CFEG-5eV-withoutOA micrographs - multiframe EER EER 3160 1113 1 1113 BIT 4096 0.727 4096 0.727

Same cautions as "CFEG, Falcon4, with 5 eV filter" apply. Please use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.

XFEG, Falcon3, without filter /data/XFEG-Falcon3 micrographs - multiframe TIFF TIFF 1501 60 1 60 SIGNED 16 BIT INTEGER 4096 0.827 4096 0.827

The pixel size in the image header (0.8583 A/px) is wrong. The processing was started with a nominal pixel size of 0.83 A/px, which was later calibrated to 0.827 A/px. Dose rate: 0.93 e/A2/frame

XFEG, Falcon4, with filter but retracted slit /data/XFEG-noslit micrographs - multiframe TIFF TIFF 1244 60 1 60 UNSIGNED 16 BIT INTEGER 4096 0.735 4096 0.735

The pixel size in the header (1.00 A/px) is wrong. Please ignore it. Dose rate: 0.44 e/A2/frame

XFEG, Falcon4, with 3 eV filter /data/XFEG-3eV micrographs - multiframe TIFF TIFF 1952 60 1 60 UNSIGNED 16 BIT INTEGER 4096 0.735 4096 0.735

Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.

XFEG, Falcon4, with 5 eV filter /data/XFEG-5eV micrographs - multiframe TIFF TIFF 1556 60 1 60 UNSIGNED 16 BIT INTEGER 4096 0.735 4096 0.735

Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.

XFEG, Falcon4, with 3 eV filter /data/XFEG-10eV micrographs - multiframe TIFF TIFF 1268 60 1 60 UNSIGNED 16 BIT INTEGER 4096 0.735 4096 0.735

Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.

XFEG, K3, without filter /data/XFEG-K3 micrographs - multiframe TIFF TIFF 1508 41 1 41 UNSIGNED BYTE 11520 0.32741 8184 0.32741

The pixel size in the image header is wrong. The processing was started with a nominal (super-resolution) pixel size of 0.325 A/px, which was later calibrated to 0.32741 A/px. Please use the provided gain reference (gain.mrc) without any transformation. Dose rate: 0.96 e/A2/frame