REL 2020-12-16 2021-05-25 2022-02-15 0000-0002-7281-8579 Hao Wu HARVARD MEDICAL SCHOOL Boston MA United States 02115 0000-0002-7281-8579 Hao Wu HARVARD MEDICAL SCHOOL Boston MA United States 02115 Sharif H Hollingsworth LR Griswold AR Hsiao JC Wang Q Bachovchin DA Wu H 967.9 10.6019/EMPIAR-10600 EMDB EMD-22974 Sharif H Hollingsworth LR Griswold AR Hsiao JC Wang Q Bachovchin DA Wu H Immunity Immunity 7 54 1392 1404.e10 2021 English 10.1016/j.immuni.2021.04.024 34019797 Unaligned multi frame micographs of CARD8-DPP9-S297A-TILT /data/CARD_DPP9_S297A_Raw_Movies_TILT micrographs - multiframe TIFF TIFF 3840 50 UNSIGNED BYTE variable 0.83 variable 0.83

For CARD8(S297A)-DPP9, 3,840 movies were collected at a stage tilt of 37° with defocus range between −0.8 to −2.5 μm and to record one shot for each of the four holes per stage movement through image shift. All movies were exposed with a total dose of 63.67 e-/Å2 for 1.5 s fractionated over 50 frames. References are in references folder