REL
2020-12-16
2021-04-14
2021-06-30
DNA origami signposts for identifying proteins on cell membranes by electron cryotomography
0000-0001-9578-4263
Lindsay
A
Baker
Division of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics
Roosevelt Drive
Oxford
Oxfordshire
United Kingdom
OX3 7BN
0000-0002-4788-2691
Kay
Grünewald
Centre for Structural Systems Biology, Heinrich-Pette-Institut, Leibniz-Institut für Experimentelle Virologie
c/o DESY, Building 15, Notkestrasse 85
Hamburg
Germany
22607
Silvester E
Vollmer B
Pražák V
Vasishtan D
Machala EA
Whittle C
Black S
Bath J
Turberfield AJ
Grünewald K
Baker LA
38.8
10.6019/EMPIAR-10613
EMDB
EMD-12188
Silvester E
Vollmer B
Pražák V
Vasishtan D
Machala EA
Whittle C
Black S
Bath J
Turberfield AJ
Grünewald K
Baker LA
DNA origami signposts for identifying proteins on cell membranes by electron cryotomography
Cell
Cell
United Kingdom
4
184
1110
1121.e16
2021
English
10.1016/j.cell.2021.01.033
33606980
Tomograms of mammlian cell derived extracellular vesicles with sfGFP-glycoprotein B from HSV-1 bound to signpost structures made by DNA origami
Tomograms of sfGFP-glycoprotein B vesicles with DNA origami SPOTs
/data
tilt series
MRC
MRC
2
1
SIGNED 16 BIT INTEGER
variable
16.32
variable
16.32
Tilt series were phase flipped and binned 2x with IMOD prior to reconstruction. Tomograms were then binned a further 3x and bandpass filtered between 2000 and 300 Å with Bsoft.