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        <keyDates>
            <depositionDate>2021-01-13</depositionDate>
            <releaseDate>2021-11-01</releaseDate>
            <updateDate>2022-02-25</updateDate>
        </keyDates>
        <title>Cryo Soft X-ray data for tetraspeck correlation</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-9395-8108</authorORCID>
            <firstName>Johannes</firstName>
            <lastName>Groen</lastName>
            <organization type="academic">Mistral Beamline Alba Synchrotron</organization>
            <street>Carrer de la Llum, 2- 26</street>
            <townOrCity>Cerdanyola del Valles</townOrCity>
            <stateOrProvince>Barcelona</stateOrProvince>
            <country>Spain</country>
            <postOrZipCode>08290</postOrZipCode>
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        <principalInvestigator private="true">
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            <firstName>Eva</firstName>
            <lastName>Pereiro</lastName>
            <organization type="academic">Mistral Beamline Alba Synchrotron</organization>
            <street>Carrer de la Llum, 2- 26</street>
            <townOrCity>Cerdanyola del Valles</townOrCity>
            <stateOrProvince>Barcelona</stateOrProvince>
            <country>Spain</country>
            <postOrZipCode>08290</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-9395-8108">Groen J</author>
            <author authorORCID="0000-0001-7626-5935">Pereiro E</author>
        </authorsList>
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        <entryDOI>10.6019/EMPIAR-10615</entryDOI>
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    <crossReferences>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-8779-8673" order="1">Okolo CA</author>
                    <author authorORCID="0000-0001-9877-0457" order="2">Kounatidis I</author>
                    <author authorORCID="0000-0002-9395-8108" order="3">Groen J</author>
                    <author authorORCID="0000-0003-3501-8473" order="4">Nahas KL</author>
                    <author order="5">Balint S</author>
                    <author authorORCID="0000-0001-7125-4137" order="6">Fish TM</author>
                    <author order="7">Koronfel MA</author>
                    <author order="8">Cortajarena AL</author>
                    <author authorORCID="0000-0002-5531-5865" order="9">Dobbie IM</author>
                    <author authorORCID="0000-0001-7626-5935" order="10">Pereiro E</author>
                    <author authorORCID="0000-0001-8091-9057" order="11">Harkiolaki M</author>
                    <title>Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures</title>
                    <journal>Nature protocols</journal>
                    <journalAbbreviation>Nat Protoc</journalAbbreviation>
                    <country></country>
                    <issue>6</issue>
                    <volume>16</volume>
                    <firstPage>2851</firstPage>
                    <lastPage>2885</lastPage>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41596-021-00522-4</externalReferences>
                    <externalReferences type="pubmed">33990802</externalReferences>
                </journalCitation>
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        <relatedBioStudiesEntries>
            <bioStudiesEntry>S-BSST576</bioStudiesEntry>
        </relatedBioStudiesEntries>
    </crossReferences>
    <imageSet>
        <name>Cryo soft X-ray tomography of Tetraspecks on Quantifoil</name>
        <directory>/data/SXT/fidtomo</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>2</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>984</imageWidth>
            <pixelWidth>100.0</pixelWidth>
            <imageHeight>974</imageHeight>
            <pixelHeight>100.0</pixelHeight>
        </dimensions>
        <details>Tetraspeck (100 and 200 nm) on Quantifoil with some 100 nm Au fiducial markers (BBI solutions). hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm</details>
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        <pickedParticlesDirectory></pickedParticlesDirectory>
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    <imageSet>
        <name>Cryo Soft X-ray tomography of NIH-3T3 cells with Tetraspeck as correlative fiducial markers</name>
        <directory>/data/SXT/fidtomo</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>2</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>984</imageWidth>
            <pixelWidth>100.0</pixelWidth>
            <imageHeight>974</imageHeight>
            <pixelHeight>100.0</pixelHeight>
        </dimensions>
        <details>Cryo Soft X-ray tomography of NIH-3T3 cells with Tetraspeck (200 nm) as correlative fiducial markers . hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm</details>
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    <imageSet>
        <name>Cryo Soft X-ray tomography of NIH-3T3 cells for mitochondrial correlation</name>
        <directory>/data/SXT/mito</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>2</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>984</imageWidth>
            <pixelWidth>100.0</pixelWidth>
            <imageHeight>974</imageHeight>
            <pixelHeight>100.0</pixelHeight>
        </dimensions>
        <details>Cryo Soft X-ray tomography of NIH-3T3 cells. hdf5 file is the raw data, mrc file is the normalized, deconvolved and reconstructed (SIRT 30 iterations) tiltseries.

Pixelsize 10x10 nanometer, ZP 25nm</details>
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        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
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