REL 2021-01-15 2021-02-12 2022-02-25 0000-0002-9395-8108 Johannes Groen Mistral Beamline Alba Synchrotron Carrer de la Llum, 2- 26 Cerdanyola del Valles Barcelona Spain 08290 0000-0001-7626-5935 Eva Pereiro Mistral Beamline Alba Synchrotron Carrer de la Llum, 2- 26 Cerdanyola del Valles Barcelona Spain 08290 Groen J Pereiro E 6.8 10.6019/EMPIAR-10624 CLXM Okolo CA Kounatidis I Groen J Nahas KL Balint S Fish TM Koronfel MA Cortajarena AL Dobbie IM Pereiro E Harkiolaki M Nature protocols Nat Protoc 6 16 2851 2885 2021 English 10.1038/s41596-021-00522-4 33990802 S-BSST576 Cryo soft X-ray tomography reconstruction of NIH-3T3 cells /data tilt series MRC MRC 3 1 32 BIT FLOAT 984 100.0 974 100.0

Data name :20200311_tomo04_3DS30_man.mrc Collection date: 11/03/2020 Location: Mistral Beamline, Alba Synchrotron (Spain) Sample: NIH-3T3 (CRL-1658) Fluorophore: Mitotracker Red (CMXRos) exogenous administration Fiducials: 100 nm Au Fiducial (BBI Solutions) Support: Quantifoil on Au Finder grid (3 mm F2/2) 520 eV; 25 nm Zoneplate; 984 * 974 Pixel; 10 * 10 * 10 nm Voxel size -65 to 65 degree rotation with steps of 1 degree (131 images). Data is already normalized and deconvolved using the standard Mistral prep-processing pipeline Alignment of the Raw data done using IMOD, reconstruction done using tomo3D with 30 iterations of SIRT

Cryo Soft X-ray tomography of NIH-3T3 cells with Tetraspeck as correlative fiducial markers /data tilt series MRC MRC 1 1 32 BIT FLOAT 984 100.0 974 100.0

Data name :20200312_tomo23_3DS30_man.mrc Collection date: 12/03/2020 Location: Mistral Beamline, Alba Synchrotron (Spain) Sample: NIH-3T3 (CRL-1658) Fluorophore: Mitotracker Red (CMXRos) exogenous administration Fiducials: 100 nm Au Fiducial (BBI Solutions) and 200 nm Tetraspeck Support: Quantifoil on Au Finder grid (3 mm F2/2) 520 eV; 25 nm Zoneplate; 984 * 974 Pixel; 10 * 10 * 10 nm Voxel size -65 to 65 degree rotation with steps of 1 degree (131 images). Data is already normalized and deconvolved using the standard Mistral prep-processing pipeline Alignment of the Raw data done using IMOD, reconstruction done using tomo3D with 30 iterations of SIRT

Cryo Soft X-ray tomography of Tetraspeck on Quantifoil /data tilt series MRC MRC 1 1 32 BIT FLOAT 984 100.0 974 100.0

Data name :20200311_tomo03_3DS30_man.mrc Collection date: 11/03/2020 Location: Mistral Beamline, Alba Synchrotron (Spain) Sample: Tetraspeck on quantifoil Fiducials: 100 nm Au Fiducial (BBI Solutions) Support: Quantifoil on Au Finder grid (3 mm F2/2) 520 eV; 25 nm Zoneplate; 984 * 974 Pixel; 10 * 10 * 10 nm Voxel size -65 to 65 degree rotation with steps of 1 degree (131 images). Data is already normalized and deconvolved using the standard Mistral prep-processing pipeline Alignment of the Raw data done using IMOD, reconstruction done using tomo3D with 30 iterations of SIRT