REL 2021-05-11 2022-06-14 2022-06-14 0000-0003-0293-1063 Juraj Ahel IMP Vienna Campus-Vienna-Biocenter 1 Vienna Vienna Austria 1030 0000-0003-1582-6924 Tim Clausen IMP Vienna Vienna Austria 1030 Ahel J Weis F Clausen T 2.1 10.6019/EMPIAR-10711 EMDB molecule EMD-12931 Ahel J Clausen T Virdee S Fletcher AJ Grabarczyk DB Deszcz L Lehner A Roitinger E BiorXiv unaligned multi-frame micrographs /data/raw_micrographs micrographs - multiframe TIFF TIFF 4591 40 1 40 UNSIGNED BYTE 11520 0.536 8184 0.536

Contents are as follows: raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected == 201216_struct_A_GainRef.mrc == gain reference for the dataset micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs micrographs_ctf_motioncorr_metadata/ == metadata of the motion correction step == cryolo_PhosaurusNet_RNF213_config.h5 == cryolo network model used for initial particles picking cryolo_PhosaurusNet_RNF213_config.json == config file for the cryolo network model used for initial particles picking coordinates_initial_cryolo/ == particle coordinates picked by cryolo coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1 refined_particles.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment == mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset

data/micrographs_ctf_motioncorr/*mrc data/refined_particles.star data/particles