<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10725" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-05-18</depositionDate>
            <releaseDate>2021-06-11</releaseDate>
            <updateDate>2021-06-11</updateDate>
        </keyDates>
        <title>Single particle cryo EM dataset of SARS-CoV-2 Spike protein with D614G substitution</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-6748-9339</authorORCID>
            <firstName>Donald</firstName>
            <lastName>Benton</lastName>
            <organization type="academic">Francis Crick Institute</organization>
            <street>Midland Road</street>
            <townOrCity>London</townOrCity>
            <country>United Kingdom</country>
            <postOrZipCode>NW1 1AT</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-5331-639X</authorORCID>
            <firstName>Steven</firstName>
            <lastName>Gamblin</lastName>
            <organization type="academic">Francis Crick Institute</organization>
            <street>Midland Road</street>
            <townOrCity>London</townOrCity>
            <country>United Kingdom</country>
            <postOrZipCode>NW1 1AT</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-6748-9339">Benton DJ</author>
            <author authorORCID="0000-0002-6680-5587">Wrobel AG</author>
            <author authorORCID="0000-0002-0387-2862">Rosenthal PB</author>
            <author authorORCID="0000-0001-5331-639X">Gamblin SJ</author>
        </authorsList>
        <datasetSize units="TB">9.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-10725</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-12229</emdbEntry>
            <emdbEntry>EMD-12230</emdbEntry>
            <emdbEntry>EMD-12231</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-6748-9339" order="1">Benton DJ</author>
                    <author authorORCID="0000-0002-6680-5587" order="2">Wrobel AG</author>
                    <author order="3">Roustan C</author>
                    <author order="4">Borg A</author>
                    <author order="5">Xu P</author>
                    <author order="6">Martin SR</author>
                    <author authorORCID="0000-0002-0387-2862" order="7">Rosenthal PB</author>
                    <author authorORCID="0000-0002-8519-9404" order="8">Skehel JJ</author>
                    <author authorORCID="0000-0001-5331-639X" order="9">Gamblin SJ</author>
                    <title>The effect of the D614G substitution on the structure of the spike glycoprotein of SARS-CoV-2</title>
                    <journal>Proceedings of the National Academy of Sciences of the United States of America</journal>
                    <journalAbbreviation>Proc Natl Acad Sci U S A</journalAbbreviation>
                    <country></country>
                    <issue>9</issue>
                    <volume>118</volume>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1073/pnas.2022586118</externalReferences>
                    <externalReferences type="pubmed">33579792</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of SARS-CoV-2 Spike with D614G substitution</name>
        <directory>/data/Images-Disc1</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>10051</numImagesOrTiltSeries>
        <framesPerImage>32</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>0.85</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.85</pixelHeight>
        </dimensions>
        <details>Uncorrected movies of D614G SARS-CoV-2 spike used to calculate closed, 1 erect RBD and 2 erect RBD forms of this mutant spike. Images were taken on FEI Krios G2 equipped with a Falcon 3 operating in electron counting mode. Images were 40s exposures fractionated into 32 frame gain corrected mrc files, with an accumulated dose of 36.8 e/Å^2. Images were collected with nominal defocus of 1 - 3 µm applied.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Images-Disc1/GridSquare_*/Data/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
