REL 2021-06-21 2023-07-25 2023-07-25 Single-particle cryo-EM dataset of the complex between ALC1 and a PARylated nucleosome 0000-0003-3405-6021 Guillaume Gaullier Uppsala University Husargatan 3, Box 596 Uppsala Sweden 751 24 0000-0001-6807-8654 Sebastian Deindl Uppsala University Husargatan 3, Box 596 Uppsala Sweden 751 24 Bacic L Gaullier G Deindl S 14.9 10.6019/EMPIAR-10739 EMDB molecule EMD-13065 EMD-13070 Bacic L Gaullier G Sabantsev A Lehmann LC Brackmann K Dimakou D Halic M Hewitt G Boulton SJ Deindl S Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome. eLife Elife 10 2021 English 10.7554/elife.71420 34486521 Bacic L Gaullier G Sabantsev A Lehmann LC Brackmann K Dimakou D Halic M Hewitt G Boulton SJ Deindl S Structure and dynamics of the chromatin remodeler ALC1 bound to a PARylated nucleosome bioRxiv 2021 10.1101/2021.06.18.448936 Unaligned movies of the complex between ALC1 and a PARylated nucleosome - batch 1 /data/batch-1 micrographs - multiframe TIFF TIFF 14502 40 1 40 UNSIGNED BYTE 5760 0.84 4092 0.84
40 gain-corrected frames per movie, collected on Titan Krios with K3 detector in counting mode.
data/batch-1/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff Unaligned movies of the complex between ALC1 and a PARylated nucleosome - batch 2 /data/batch-2 micrographs - multiframe TIFF TIFF 19496 40 1 40 UNSIGNED BYTE 5760 0.84 4092 0.84
40 gain-corrected frames per movie, collected on Titan Krios with K3 detector in counting mode.
data/batch-2/Images-Disc1/GridSquare_*/Data/FoilHole_*_fractions.tiff Extracted particles at original pixel size - large set /data/particles picked particles - multiframe - processed MRCS MRCS 426066 1 32 BIT FLOAT 512 0.84 512 0.84
Large set of particles, homogeneous in composition/stoichiometry but highly heterogeneous in conformation, as assessed by RELION Refine3D.
data/particles/j119-particles-0.84Apix-box512.star data/particles Extracted particles at original pixel size - smaller subset /data/particles picked particles - multiframe - processed MRCS MRCS 43698 1 32 BIT FLOAT 512 0.84 512 0.84
Smaller subset of particles, homogeneous in composition/stoichiometry but heterogeneous in conformation, as assessed by RELION Refine3D. Conformational heterogeneity was tractable using cryoSPARC 3DVA and cryoDRGN.
data/particles/j167-particles-0.84Apix-box512.star data/particles