Venous puncture wound thrombi, 1, 5, 20 min post-puncture, 100 nm XY raw images, 20 nm XY pixels every 20 micons, 3 nm wide area TEM montages at selected depth

REL 2021-07-22 2023-05-22 2023-05-23 Venous puncture wound thrombi, 1, 5, 20 min post-puncture, 100 nm XY raw images, 20 nm XY pixels every 20 micons, 3 nm wide area TEM montages at selected depth 0000-0002-4644-2504 Brian Storrie Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences 4301 West Markham Street Little Rock Arkansas United States 72205 0000-0002-4644-2504 Brian Storrie Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences 4301 West Markham Street Little Rock Arkansas United States 72205 Storrie B Leapman RD National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) R01HL119393 R56HL19393 R01HL155519 United States 381.2 10.6019/EMPIAR-10785 SBF-SEM tissue Rhee SW Pokrovskaya ID Ball KK Ling K Vedanaparti Y Cohen J Cruz DRD Zhao OS Aronova MA Zhang G Kamykowski JA Leapman RD Storrie B Rogers ER Venous puncture wound hemostasis results in a vaulted thrombus structured by locally nucleated platelet aggregates Communications biology Commun Biol 1 4 2021 English 10.1038/s42003-021-02615-y 34531522

The micrographs are the raw images from collecting individual 3View slices at a 100 nm by 100 nm XY pixel size with a Z step size of 200 nm for mouse jugular vein punture wound made by a 30 gauge needle at 1, 5 or 20 min post puncture. Samples were chemically fixed with paraformaldehyde and later with glutaraldehyde and stained for serial block face imaging pre-embedding in plastic.

SBF-SEM imaging of jugular vein thrombi, 30 gauge needle puncture wounds, at 20 nm and 100 pixel, 20 micron and 200 nm step size, and 3 nm pixel wide TEM at ~mid-thrombus across full width montage /data/20 nm SBF-SEM 20 nm XY 20 micrron step spacing Storrie micrographs - multiframe DM4 DM4 15 1 UNSIGNED 16 BIT INTEGER 12500 20.0 20000 20.0

SBF-SEM image sets of individual jugular vein puncture wound thrombi (clots) at 1, 5, and 20 min post puncture. 20 nm image sets are at 20,20 nm XY and 20 micron Z step. 100 nm image sets are at 100,100 nm XY and 200 nm spacing. 20 nm sets are 10-30 images each and 100 nm sets are often about 2500 images each.