REL
2021-07-14
2021-11-01
2021-11-01
electron cryo-tomograms of axons from human cerebral organoids, expressing L1CAM-GFP
0000-0002-2778-3936
Wanda
Kukulski
University of Bern, Institute of Biochemistry and Molecular Medicine
Bühlstrasse 28
Bern
N/A
Switzerland
3011
0000-0002-2778-3936
Wanda
Kukulski
University of Bern, Institute of Biochemistry and Molecular Medicine
Bühlstrasse 28
Bern
N/A
Switzerland
3011
0000-0003-2324-8853
Madeline
A
Lancaster
MRC Laboratory of Molecular Biology
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Hoffmann P
Giandomenico S
Ganeva I
Wozny M
Sutcliffe M
Lancaster M
Kukulski W
30.5
10.6019/EMPIAR-10805
EMDB
EMD-13196
Hoffmann P
Giandomenico S
Ganeva I
Wozny M
Sutcliffe M
Lancaster M
Kukulski W
Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids
eLife
Elife
10
2021
English
10.7554/eLife.70269
reconstructed electron tomographic volumes of axons from human cerebral organoids expressing L1CAM-GFP
/data/tomograms
reconstructed volumes
MRC
MRC
8
1
SIGNED BYTE
1920
7.4
1854
7.4
Tomograms were reconstructed with IMOD using back projection with a SIRT-like filter from stacks aligned by patch tracking, binning 2.
raw image frames of tilt series acquired on axons from human cerebral organoids expressing L1CAM-GFP
/data/raw_data
micrographs - multiframe
TIFF
TIFF
8
4
UNSIGNED 16 BIT INTEGER
3710
3.7
3838
3.7
8 folders, each corresponding to one tilt series and containing raw image frames as well as metadata in an .mdoc file. The image frames are not aligned and not gain-corrected. An additional folder contains the gain reference and defect file.