REL 2021-07-28 2021-12-17 2021-12-17 0000-0002-4691-5746 Helen Elizabeth Foster Human Technopole Viale Rita Levi‑Montalcini, 1 Milan Italy 20157 0000-0001-7292-5430 Andrew Carter MRC Laboratory of Molecular Biology Cambridge Biomedical Campus, Francis Crick Ave, Cambridge Cambridgeshire United Kingdom CB2 0QH Foster HE Ventura Santos C Carter AP 200.8 10.6019/EMPIAR-10815 EMDB EMD-13600 EMD-13601 EMD-13602 Foster HE Ventura Santos C Carter AP Journal of Cell Biology J. Cell Biol. United Kingdom 2 221 2021 English 10.1083/jcb.202103154 34878519 Tomograms of mouse dorsal root ganglion axons (dataset 1) binned by 4 and deconvolved /data/bin4_deconvolved_tomos reconstructed volumes MRC MRC 30 1 32 BIT FLOAT 927 13.76 927 13.76

Reconstructed bin4 tomograms. Volumes were filtered using the Wiener-like deconvolution filter implemented in WARP (Tegunov 2018, https://github.com/dtegunov/tom\_deconv). Tomograms were reconstructed in IMOD by weighted back projection.

Corrected, aligned, dose-filtered and order-sorted tilt series for mouse dorsal root ganglion axons (dataset 1) /data/Dose-filt_stacks tilt series MRC MRC 30 61 32 BIT FLOAT 3710 3.44 3710 3.44

Corrected, aligned, dose-filtered and sorted tilt series. The tilt series were generated from the raw frame images by correction for gain, defects and motion. In addition, tilt images were dose-filtered. Gain-correction and defects files are provided in the Raw_frames folder. Motion-correction was performed with alignframes (IMOD) operated in subTOM.