REL 2021-12-22 2022-01-12 2022-01-12 0000-0003-1750-4793 Melanie Ohi University of Michigan, Life Sciences Institute 210 Washtenaw Ave Ann Arbor MI United States 48109 0000-0003-1750-4793 Melanie Ohi University of Michigan, Life Sciences Institute 210 Washtenaw Ave Ann Arbor MI United States 48109 0000-0002-0049-1079 James Crowe Department of Pathology, Microbiology and Immunology, Vanderbilt University 2213 Garland Ave Nashville TN United States 37232-0417 Williamson LE Gilliland Jr T Yadav PK Binshtein E Bombardi R Kose N Nargi RS Sutton RE Durie CL Armstrong E Carnahan RH Walker LM Kim AS Fox JM Diamond MS Ohi MD Klimstra WB Crowe Jr JE 111.8 10.6019/EMPIAR-10890 EMDB EMD-22223 Williamson LE Gilliland T Yadav PK Binshtein E Bombardi R Kose N Nargi RS Sutton RE Durie CL Armstrong E Carnahan RH Walker LM Kim AS Fox JM Diamond MS Ohi MD Klimstra WB Crowe JE Cell Cell 7 183 1884 1900.e23 2020 English 10.1016/j.cell.2020.11.011 33301709

Collected on a Titan Krios electron microscope equipped with a K2 Summit Direct Electron Detector (Gatan). Nominal pixel size of 1.64 Å per pixel. Movies (50 frames, 200 msec exposure per frame) were corrected for beam-induced motion and dose-weighted using MotionCor2 resulting in global motion-corrected frame stacks and summed micrographs.

Motion corrected micrographs Chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-33 Antibody /data micrographs - single frame MRC MRC 2111 50 SIGNED 32 BIT INTEGER 3838 1.64 3710 1.64

Images for SINV/EEEV:EEEV-33 Fab were collected on a Titan Krios electron microscope (Thermo Fisher Scientific) equipped with a K2 Summit Direct Electron Detector operated at 300 kV and having a nominal pixel size of 1.64 Å per pixel. Micrographs were acquired automatically using Leginon software. The total exposure time was 10 s and frames were recorded every 0.2 s. Defocus values ranged from 1.0 to 2.5 μm. The total accumulated does was ~50 e−/Å2. Deposited micrographs were motion corrected using MotionCor2.