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        <keyDates>
            <depositionDate>2021-09-29</depositionDate>
            <releaseDate>2022-02-07</releaseDate>
            <updateDate>2022-02-07</updateDate>
        </keyDates>
        <title>Cryo electron microscopy SMARCAD1 with nucleosome in ADP-BeF3 bound state</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-3292-2379</authorORCID>
            <firstName>Karolin</firstName>
            <lastName>Luger</lastName>
            <organization type="academic">Department of Biochemistry University of Colorado Boulder</organization>
            <street>N/A</street>
            <townOrCity>Boulder</townOrCity>
            <stateOrProvince>Colorado</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>80303</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-5136-5331</authorORCID>
            <firstName>Karolin</firstName>
            <lastName>Luger</lastName>
            <organization type="academic">Department of Biochemistry University of Colorado Boulder</organization>
            <townOrCity>Boulder</townOrCity>
            <country>United States</country>
            <postOrZipCode>80303</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-3292-2379">Markert JW</author>
            <author authorORCID="0000-0001-5136-5331">Luger K</author>
        </authorsList>
        <datasetSize units="TB">6.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-10939</entryDOI>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-25180</emdbEntry>
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        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-3292-2379" order="1">Markert J</author>
                    <author order="2">Zhou K</author>
                    <author authorORCID="0000-0001-5136-5331" order="3">Luger K</author>
                    <title>SMARCAD1 is an ATP-dependent histone octamer exchange factor with de novo nucleosome assembly activity</title>
                    <journal>Science advances</journal>
                    <journalAbbreviation>Sci Adv</journalAbbreviation>
                    <country>United States</country>
                    <issue>42</issue>
                    <volume>7</volume>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1126/sciadv.abk2380</externalReferences>
                    <externalReferences type="pubmed">34652950</externalReferences>
                    <details>Raw micrographs (pixel size = 1.065 angstroms, 60 frames with total dose of 60 electrons/micrograph)</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of SMARCAD1-nucleosome with ADP-BeF3</name>
        <directory>/data/Jon_Janelia</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>11235</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>variable</imageWidth>
            <pixelWidth>1.065</pixelWidth>
            <imageHeight>variable</imageHeight>
            <pixelHeight>1.065</pixelHeight>
        </dimensions>
        <details>Mag 64k, pixel size 1.065, per frame exposure 1 electron per angstrom squared, defocus range 1.2-2.5 micrometers, total 60 frames, collected on Krios equipped with K3 detector</details>
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