REL
2022-03-29
2022-04-22
2022-04-22
Cryo-EM structure of human U2 snRNP after ATP-dependent remodeling
0000-0002-0789-2692
Jonas
Tholen
EMBL
71 Avenue des Martyrs
Grenoble
Isere
France
38042
0000-0001-8859-5229
Wojciech
P.
Galej
EMBL
71 Avenue des Martyrs
Grenoble
Isere
France
38042
Tholen J
Galej WP
Weis F
1.5
10.6019/EMPIAR-11000
EMDB
EMD-13812
Tholen J
Razew M
Weis F
Galej WP
Structural basis of branch site recognition by the human spliceosome
Science (New York, N.Y.)
Science
6576
375
50
57
2021
English
10.1126/science.abm4245
34822310
We isolated human 17S U2 snRNP and reconstituted in vitro its ATP–dependent remodeling and binding to the pre–messenger RNA substrate. We determined a series of high-resolution (2.0 to 2.2 angstrom) structures providing snapshots of the spliceosomal branch site selection process.
Unaligned multi-frame micrographs of the U2 snRNP after ATP-dependent remodeling
/data/tatatpcoll3_2103117images
micrographs - multiframe
TIFF
TIFF
7809
40
UNSIGNED BYTE
5760
0.64
4092
0.64
Unaligned movies (40 frames) acquired over 1 s with a total 50.76e/Å2 dose with defocus range of -0.8 to -1.8 μm in 0.1 μm steps. Gain reference is CountRef_114-1_001.mrc
data/tatatpcoll3_2103117images/*.tif
data/allparticlesWARPpicked.star
Final polished particles of the U2 snRNP after ATP-dependent remodeling
/data/particles
picked particles - single frame - processed
MRCS
MRCS
7611
1
32 BIT FLOAT
640
0.64
640
0.64
Polished particles after auto-refinement in Relion3.1. In the star file the columns rlnMicrographName and rlnImageName are not correct: The paths have to be changed to point to the mrc/mrcs files. The final resolution was 2.15 A. The 3' domain of U2 snRNP could not be resolved.
data/finalrefinedparticles_data.star
data/particles/*.mrcs