REL
2022-04-07
2022-05-17
2022-05-23
Conformational rearrangements upon start codon recognition in human 48S translation initiation complex
0000-0002-4609-4052
Niels
Fischer
Max-Planck-Institute for Multidisciplinary Sciences
Am Fassberg 11
Goettingen
Lower Saxony
Germany
37077
0000-0002-4609-4052
Niels
Fischer
Max-Planck-Institute for Multidisciplinary Sciences
Am Fassberg 11
Goettingen
Lower Saxony
Germany
37073
Yi SH
Petrychenko V
Schliep JE
Goyal A
Linden A
Chari A
Urlaub H
Stark H
Rodnina MV
Adio S
Fischer N
1.1
10.6019/EMPIAR-11005
EMDB
EMD-14113
EMD-14114
Yi SH
Petrychenko V
Schliep JE
Goyal A
Linden A
Chari A
Urlaub H
Stark H
Rodnina MV
Adio S
Fischer N
Conformational rearrangements upon start codon recognition in human 48S translation initiation complex.
Nucleic acids research
Nucleic Acids Res
United Kingdom
9
50
5282
5298
2022
English
10.1093/nar/gkac283
35489072
Motion-corrected, dose-weighted micrographs
/data/micrographs
micrographs - single frame
MRC
MRC
15544
1
32 BIT FLOAT
4096
1.16
4096
1.16
Micrographs were obtained from movie-stacks of 20 frames à 2.4 e/A^2 by global motion-correction and dose-weighting using MotionCor2, CTF parameters were estimated using GCTF 1.06 and particles were selected by GAUTOMATCH.
- Micrographs .star-file: "data/micrographs_gctf.star"
- Particle coordinates: "*data/micrographs/*_automatch.star"
- 3D masks used for sorting particles in 180px box at 2.32Apix: "data/mask180px_40Shead.mrc" & "data/mask180px_TCandDC.mrc"
data/micrographs/*.mrc
Particles of human 48S IC in open state ("open")
/data/particles_48SIC_open
picked particles - single frame - processed
MRCS
MRCS
57148
1
32 BIT FLOAT
360
1.16
360
1.16
Particles .star-file & 3D mask for final 3D refinement of open state (EMD-14113): "data/particles_48SIC_open.star" & "data/mask360px_48SIC_open.mrc"
data/micrographs/*.mrc
data/particles_48SIC_open/*.mrcs
data/particles_48SIC_open
Particles of human 48S IC in closed state ("closed")
/data/particles_48SIC_closed
picked particles - single frame - processed
MRCS
MRCS
364950
1
32 BIT FLOAT
360
1.16
360
1.16
Particles .star-file & 3D mask for final 3D refinement of closed state (EMD-14114): "data/particles_48SIC_closed.star" & "data/mask360px_48SIC_closed.mrc"
data/micrographs/*.mrc
data/particles_48SIC_closed/*.mrcs
data/particles_48SIC_closed