REL 2022-04-28 2022-05-10 2022-05-10 0000-0001-7982-6494 Kedar Narayan NCI/FNLCR Frederick MD United States 21701 0000-0001-7982-6494 Kedar Narayan NCI/FNLCR Frederick MD United States 21701 Narayan K Conrad RW 2.8 10.6019/EMPIAR-11037 FIB-SEM Narayan K EMPIAR-11035 Images and masks correpsonding to grayscale 2D EM image patches and paired mitochondrial label maps /data micrographs - single frame TIFF TIFF 43720 1 UNSIGNED BYTE 224 variable 224 variable

PLEASE READ! There are 21,860 annotated images in CEM-MitoLab. They contain 135,285 mitochondrial instances in total. The images are a subset of CEM1.5M (also on EMPIAR; EMPIAR-11035). The image patches are mostly 224 x 224 pixels, however some are 512 x 512, and some are smaller. The directory names are either randomized for in-house or unpublished data, or kept as-is for published data. Each directory has "image" and "mask" subdirectory. These directories are populated by grayscale images and "paired" mitochondrial instance label map files, respectively. Both are unsigned byte tiff files. For the mito labels, background is 0, individual instances per image are 1,2,3...255. 2D or 3D = dimensionality of the original EM data LOC-0,1,2 = is the index along z, y, x axis (ie. the numbers following are the location in the original image). Extents of 5, m, n, when present, is because images were present as a flipbook of 5. Only the middle one is annotated and uploaded here with the paired grayscale images. For practical purposes these can be ignored. PLEASE SEE .XLS FILE <</metadata/cem_mitolab_metadata.xlsx>> FOR METADATA USING REMBI PRINCIPLES (Sarkans et al Nature Methods 2021)