<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11043" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-02-08</depositionDate>
            <releaseDate>2022-08-08</releaseDate>
            <updateDate>2022-09-09</updateDate>
        </keyDates>
        <title>Architecture of the human erythrocyte ankyrin-1 complex</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-1876-196X</authorORCID>
            <firstName>Oliver</firstName>
            <middleName>Biggs</middleName>
            <lastName>Clarke</lastName>
            <organization type="academic">Department of Anesthesiology, Columbia University</organization>
            <townOrCity>New York</townOrCity>
            <country>United States</country>
            <postOrZipCode>10032</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0003-1876-196X</authorORCID>
            <firstName>Oliver</firstName>
            <middleName>Biggs</middleName>
            <lastName>Clarke</lastName>
            <organization type="academic">Department of Anesthesiology, Columbia University</organization>
            <townOrCity>New York</townOrCity>
            <country>United States</country>
            <postOrZipCode>10032</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-4524-6605">Vallese F</author>
            <author authorORCID="0000-0003-1876-196X">Clarke OB</author>
        </authorsList>
        <datasetSize units="TB">5.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-11043</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-26943</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-4524-6605" order="1">Vallese F</author>
                    <author authorORCID="0000-0001-9915-0145" order="2">Kim K</author>
                    <author authorORCID="0000-0002-4086-0895" order="3">Yen LY</author>
                    <author authorORCID="0000-0003-3060-7738" order="4">Johnston JD</author>
                    <author authorORCID="0000-0001-8634-2279" order="5">Noble AJ</author>
                    <author authorORCID="0000-0002-8901-1659" order="6">Calì T</author>
                    <author authorORCID="0000-0003-1876-196X" order="7">Clarke OB</author>
                    <title>Architecture of the human erythrocyte ankyrin-1 complex</title>
                    <journal>Nature structural &amp; molecular biology</journal>
                    <journalAbbreviation>Nat Struct Mol Biol</journalAbbreviation>
                    <country></country>
                    <issue>7</issue>
                    <volume>29</volume>
                    <firstPage>706</firstPage>
                    <lastPage>718</lastPage>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41594-022-00792-w</externalReferences>
                    <externalReferences type="pubmed">35835865</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of ankyrin-1 complex</name>
        <directory>/data/movies</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>14926</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>The micrographs are of 50 raw movie frames accumulating 58.2 electrons per squared Angstrom in a 2.5 second exposure.
Particle positions are in .star files with X and Y coordinates in 3rd and 4th column respectively.

consensus_particles_stack.mrcs contains the particle images of the ankyrin-1 complex consensus particle set.

Band3_GPA_particles.star contains the particle coordinates and CTF parameters of the band3-GPA partcle set.</details>
        <segmentationList/>
        <micrographsFilePattern>data/movies/*.tif</micrographsFilePattern>
        <pickedParticlesFilePattern>data/consensus_particles/consensus_particles_stack.mrcs</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/consensus_particles</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Aligned micrographs of ankyrin-1 complex</name>
        <directory>/data/motion_corrected</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>14926</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>The raw movie frames are aligned with patch motion correction in cryoSPARC. (50 frames, total dose: 58.2e-/A2, Cs=2.7)
Particle positions are in .star files with X and Y coordinates in 3rd and 4th column respectively.
consensus_particles_stack.mrcs contains the particle images of the ankyrin-1 complex consensus particle set.
Band3_GPA_particles.star contains the particle coordinates and CTF parameters of the band3-GPA partcle set.</details>
        <segmentationList/>
        <micrographsFilePattern>data/motion_corrected/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/consensus_particles/consensus_particles_stack.mrcs</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/consensus_particles</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Consensus particle stack for the ankyrin-1 complex</name>
        <directory>/data/consensus_particles</directory>
        <category>picked particles - single frame - unprocessed</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>710437</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>450</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>450</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>710437 particle image stack is saved as consensus_particles_stack.mrcs

Particle coordinates and CTF parameters are saved in consensus_particles_stack.star

Pixel size: 0.83 Å/pix
Box size: 450 pix</details>
        <segmentationList/>
        <micrographsFilePattern>data/motion_corrected/*mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/consensus_particles/consensus_particles_stack.mrcs</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/consensus_particles</pickedParticlesDirectory>
    </imageSet>
</entry>
