REL 2022-05-09 2022-06-01 2022-06-01 0000-0003-4939-4170 Kimberly Meechan European Molecular Biology Laboratory, Heidelberg, Germany Meyerhofstraße 1 Heidelberg Baden-Württemberg Germany 69117 0000-0001-8027-1836 Yannick Schwab European Molecular Biology Laboratory, Heidelberg, Germany Meyerhofstraße 1 Heidelberg Baden-Württemberg Germany 69117 0000-0003-0260-613X Lucy Collinson Francis Crick Institute, London, UK 1 Midland Road London Greater London United Kingdom NW1 1AT Meechan K Guan W Riedinger A Stankova V Yoshimura A Pipitone R Milberger A Schaar H Romero-Brey I Templin R Peddie C J Schieber N L Jones M L Collinson L Schwab Y 151.0 10.6019/EMPIAR-11055 Hard X-ray/X-ray microCT Meechan K Guan W Riedinger A Stankova V Yoshimura A Pipitone R Milberger A Schaar H Romero-Brey I Templin R Peddie C J Schieber N L Jones M L Collinson L Schwab Y BioRxiv BioRxiv 2022 English sample_a_before_targeting.tif /data/sample_a_e2 reconstructed volumes TIFF TIFF 1 479 UNSIGNED BYTE 1000 variable 660 variable

Micro-CT of sample a before targeting (ready for crosshair)

sample_a_after_targeting.tif /data/sample_a_e2 reconstructed volumes TIFF TIFF 1 465 UNSIGNED BYTE 738 variable 870 variable

Micro-CT of sample a after targeting (ready for accuracy measures)

Raw sample a micro-CT - before reconstruction and before targeting /data/sample_a_e2/raw_images/before_targeting reconstructed volumes TIFF TIFF 939 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample a micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample a micro-CT - after reconstruction and before targeting /data/sample_a_e2/raw_images/before_targeting/Platy-A_1micron_Rec reconstructed volumes PNG PNG 479 1 UNSIGNED BYTE 2016 variable 2016 variable

Raw sample a micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample a micro-CT - before reconstruction and after targeting /data/sample_a_e2/raw_images/after_targeting reconstructed volumes TIFF TIFF 1799 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample a micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample a micro-CT - after reconstruction and after targeting /data/sample_a_e2/raw_images/after_targeting/2_1um_again_Rec reconstructed volumes PNG PNG 465 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample a micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

sample_b_before_targeting.tif /data/sample_b_e4 reconstructed volumes TIFF TIFF 1 441 UNSIGNED BYTE 1046 variable 688 variable

Micro-CT of sample b before targeting (ready for crosshair)

sample_b_after_targeting.tif /data/sample_b_e4 reconstructed volumes TIFF TIFF 1 559 UNSIGNED BYTE 1224 variable 774 variable

Micro-CT of sample b after targeting (ready for accuracy measures)

Raw sample b micro-CT - before reconstruction and before targeting /data/sample_b_e4/raw_images/before_targeting reconstructed volumes TIFF TIFF 1799 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample b micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample b micro-CT - after reconstruction and before targeting /data/sample_b_e4/raw_images/before_targeting/Sample_4_1um_Platy_Rec reconstructed volumes PNG PNG 441 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample b micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample b micro-CT - before reconstruction and after targeting /data/sample_b_e4/raw_images/after_targeting reconstructed volumes TIFF TIFF 1799 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample b micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample b micro-CT - after reconstruction and after targeting /data/sample_b_e4/raw_images/after_targeting/4_1um_Rec reconstructed volumes PNG PNG 559 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample b micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

sample_c_before_targeting.tif /data/sample_c_e6 reconstructed volumes TIFF TIFF 1 462 UNSIGNED BYTE 838 variable 568 variable

Micro-CT of sample c before targeting (ready for crosshair)

sample_c_after_targeting.tif /data/sample_c_e6 reconstructed volumes TIFF TIFF 1 430 UNSIGNED BYTE 663 variable 867 variable

Micro-CT of sample c after targeting (ready for accuracy measures)

Raw sample c micro-CT - before reconstruction and before targeting /data/sample_c_e6/raw_images/before_targeting reconstructed volumes TIFF TIFF 1799 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample c micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample c micro-CT - after reconstruction and before targeting /data/sample_c_e6/raw_images/before_targeting/Sample_6_1um_Platy_Rec reconstructed volumes PNG PNG 462 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample c micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample c micro-CT - before reconstruction and after targeting /data/sample_c_e6/raw_images/after_targeting reconstructed volumes TIFF TIFF 1799 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample c micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample c micro-CT - after reconstruction and after targeting /data/sample_c_e6/raw_images/after_targeting/6_1um_again_Rec reconstructed volumes PNG PNG 430 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample c micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

sample_d_before_targeting.tif /data/sample_d_e9 reconstructed volumes TIFF TIFF 1 414 UNSIGNED BYTE 894 variable 576 variable

Micro-CT of sample d before targeting (ready for crosshair)

sample_d_after_targeting.tif /data/sample_d_e9 reconstructed volumes TIFF TIFF 1 459 UNSIGNED BYTE 939 variable 762 variable

Micro-CT of sample d after targeting (ready for accuracy measures)

Raw sample d micro-CT - before reconstruction and before targeting /data/sample_d_e9/raw_images/before_targeting reconstructed volumes TIFF TIFF 1799 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample d micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample d micro-CT - after reconstruction and before targeting /data/sample_d_e9/raw_images/before_targeting/ESample_9_1um_Platy_Rec reconstructed volumes PNG PNG 519 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample d micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample d micro-CT - before reconstruction and after targeting /data/sample_d_e9/raw_images/after_targeting reconstructed volumes TIFF TIFF 1799 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample d micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample d micro-CT - after reconstruction and after targeting /data/sample_d_e9/raw_images/after_targeting/9_1um_Rec reconstructed volumes PNG PNG 459 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample d micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

sample_e_before_targeting.tif /data/sample_e_e13 reconstructed volumes TIFF TIFF 1 415 UNSIGNED BYTE 770 variable 706 variable

Micro-CT of sample e before targeting (ready for crosshair)

sample_e_after_targeting.tif /data/sample_e_e13 reconstructed volumes TIFF TIFF 1 443 UNSIGNED BYTE 807 variable 846 variable

Micro-CT of sample e after targeting (ready for accuracy measures)

Raw sample e micro-CT - before reconstruction and before targeting /data/sample_e_e13/raw_images/before_targeting reconstructed volumes TIFF TIFF 939 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample e micro-CT (before reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample e micro-CT - after reconstruction and before targeting /data/sample_e_e13/raw_images/before_targeting/Platy_sample_13_1um_Rec reconstructed volumes PNG PNG 463 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample e micro-CT (after reconstruction and before targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample e micro-CT - before reconstruction and after targeting /data/sample_e_e13/raw_images/after_targeting reconstructed volumes TIFF TIFF 1799 1 UNSIGNED 16 BIT INTEGER 2016 variable 1344 variable

Raw sample e micro-CT (before reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

Raw sample e micro-CT - after reconstruction and after targeting /data/sample_e_e13/raw_images/after_targeting/12_1um_Rec reconstructed volumes PNG PNG 443 1 UNSIGNED 16 BIT INTEGER 2016 variable 2016 variable

Raw sample e micro-CT (after reconstruction and after targeting) from Bruker Skyscan 1272, along with log files of scan settings

sample_f_before_targeting.tif /data/sample_f_c5 reconstructed volumes TIFF TIFF 1 997 UNSIGNED BYTE 994 variable 1015 variable

Micro-CT of sample f before targeting (ready for crosshair)

sample_f_after_targeting.tif /data/sample_f_c5 reconstructed volumes TIFF TIFF 1 997 UNSIGNED BYTE 993 variable 1015 variable

Micro-CT of sample f after targeting (ready for accuracy measures)

Raw sample f micro-CT - after reconstruction and before targeting /data/sample_f_c5/raw_images/before_targeting reconstructed volumes TIFF TIFF 1 997 UNSIGNED 16 BIT INTEGER 994 variable 1015 variable

Raw sample f micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

Raw sample f micro-CT - after reconstruction and after targeting /data/sample_f_c5/raw_images/after_targeting reconstructed volumes TIFF TIFF 1 997 UNSIGNED 16 BIT INTEGER 993 variable 1015 variable

Raw sample f micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

sample_g_before_targeting.tif /data/sample_g_c8 reconstructed volumes TIFF TIFF 1 995 UNSIGNED BYTE 992 variable 1015 variable

Micro-CT of sample g before targeting (ready for crosshair)

sample_g_after_targeting.tif /data/sample_g_c8 reconstructed volumes TIFF TIFF 1 993 UNSIGNED BYTE 1015 variable 987 variable

Micro-CT of sample g after targeting (ready for accuracy measures)

Raw sample g micro-CT - after reconstruction and before targeting /data/sample_g_c8/raw_images/before_targeting reconstructed volumes TIFF TIFF 1 995 UNSIGNED 16 BIT INTEGER 992 variable 1015 variable

Raw sample g micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

Raw sample g micro-CT - after reconstruction and after targeting /data/sample_g_c8/raw_images/after_targeting reconstructed volumes TIFF TIFF 1 993 UNSIGNED 16 BIT INTEGER 987 variable 1015 variable

Raw sample g micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

sample_h_before_targeting.tif /data/sample_h_c10 reconstructed volumes TIFF TIFF 1 996 UNSIGNED BYTE 992 variable 1015 variable

Micro-CT of sample h before targeting (ready for crosshair)

sample_h_after_targeting.tif /data/sample_h_c10 reconstructed volumes TIFF TIFF 1 996 UNSIGNED BYTE 991 variable 1015 variable

Micro-CT of sample h after targeting (ready for accuracy measures)

Raw sample h micro-CT - after reconstruction and before targeting /data/sample_h_c10/raw_images/before_targeting reconstructed volumes TIFF TIFF 1 996 UNSIGNED 16 BIT INTEGER 992 variable 1015 variable

Raw sample h micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

Raw sample h micro-CT - after reconstruction and after targeting /data/sample_h_c10/raw_images/after_targeting reconstructed volumes TIFF TIFF 1 996 UNSIGNED 16 BIT INTEGER 991 variable 1015 variable

Raw sample h micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

sample_i_before_targeting.tif /data/sample_i_c12 reconstructed volumes TIFF TIFF 1 995 UNSIGNED BYTE 992 variable 1015 variable

Micro-CT of sample i before targeting (ready for crosshair)

sample_i_after_targeting.tif /data/sample_i_c12 reconstructed volumes TIFF TIFF 1 997 UNSIGNED BYTE 1015 variable 991 variable

Micro-CT of sample i after targeting (ready for accuracy measures)

Raw sample i micro-CT - after reconstruction and before targeting /data/sample_i_c12/raw_images/before_targeting reconstructed volumes TIFF TIFF 1 995 UNSIGNED 16 BIT INTEGER 992 variable 1015 variable

Raw sample i micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

Raw sample i micro-CT - after reconstruction and after targeting /data/sample_i_c12/raw_images/after_targeting reconstructed volumes TIFF TIFF 1 997 UNSIGNED 16 BIT INTEGER 991 variable 1015 variable

Raw sample i micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

sample_j_before_targeting.tif /data/sample_j_c13 reconstructed volumes TIFF TIFF 1 997 UNSIGNED BYTE 993 variable 1015 variable

Micro-CT of sample j before targeting (ready for crosshair)

sample_j_after_targeting.tif /data/sample_j_c13 reconstructed volumes TIFF TIFF 1 996 UNSIGNED BYTE 1015 variable 986 variable

Micro-CT of sample j after targeting (ready for accuracy measures)

Raw sample j micro-CT - after reconstruction and before targeting /data/sample_j_c13/raw_images/before_targeting reconstructed volumes TIFF TIFF 1 997 UNSIGNED 16 BIT INTEGER 993 variable 1015 variable

Raw sample j micro-CT (after reconstruction and before targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.

Raw sample j micro-CT - after reconstruction and after targeting /data/sample_j_c13/raw_images/after_targeting reconstructed volumes TIFF TIFF 1 996 UNSIGNED 16 BIT INTEGER 986 variable 1015 variable

Raw sample j micro-CT (after reconstruction and after targeting) from Versa 510 X-ray microscope (Zeiss). The corresponding before reconstruction file (.txrm) is available in the same directory.