REL 2022-06-14 2022-07-18 2022-07-18 0000-0001-6768-3406 Stephen Brohawn Department of Molecular & Cell Biology, University of California Berkeley Berkeley United States 94720 0000-0001-6768-3406 Stephen Brohawn Department of Molecular & Cell Biology, University of California Berkeley Berkeley United States 94720 Dolan KA Dutta M Kern DM Kotecha A Voth GA Brohawn SG 4.0 10.6019/EMPIAR-11067 EMDB molecule EMD-26993 Dolan KA Dutta M Kern DM Kotecha A Voth GA Brohawn SG bioRxiv 2022 10.1101/2022.06.12.495841 SARS-CoV-2 M protein in an MSP1E3D1 lipid nanodisc /data micrographs - multiframe EER EER 7588 1251 BIT variable 0.3635 variable 0.3635

EER-format movies titled by total frame number. We recommend merging 25 frames for one dose fraction which gives 50 frame movies. The dose per frame is 0.02016 e/A2 (or 1.008 e/A2 per suggested fraction). The gain reference image is in the same folder with the frames, it does not need to be flipped or rotated. The super-resolution pixel size is 0.3635 A for a physical pixel size of 0.727 A.