REL
2022-05-25
2023-01-19
2023-01-19
Cryo Electron Microscopy of CHMP1B/IST1 Membrane-Bound Filaments with Brominated Lipid Probes
0000-0002-6149-6447
Frank
Russell
Moss
University of California, San Francisco
San Francisco, CA
United States
94110
0000-0003-2231-2577
Adam
Frost
University of California, San Francisco
San Francisco, CA
United States
94158
Moss FR
Lincoff J
Tucker M
Mohammed A
Grabe M
Frost A
3.7
10.6019/EMPIAR-11277
EMDB
molecule
EMD-27991
EMD-28694
EMD-28695
EMD-28696
EMD-28697
EMD-28698
EMD-28699
EMD-28700
EMD-28701
EMD-28702
EMD-28703
EMD-28704
EMD-28705
EMD-28706
EMD-28707
EMD-28708
EMD-28709
EMD-28710
EMD-28711
EMD-28712
EMD-28713
EMD-28714
EMD-28715
EMD-28716
EMD-28717
EMD-28718
EMD-28719
EMD-28722
Moss FR
Lincoff J
Tucker M
Mohammed A
Grabe M
Frost A
Frost A
Moss FR
Brominated lipid probes expose structural asymmetries in constricted membranes
Nature structural & molecular biology
Nat Struct Mol Biol
2023
English
10.1038/s41594-022-00898-1
36624348
Motion-corrected micrographs of membrane-bound CHMP1B-IST1 filaments with and without brominated lipid probes.
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 0% cholesterol
/data/0CHOL_Arctica
micrographs - single frame
MRC
MRC
615
1
32 BIT FLOAT
5760
1.14
4092
1.14
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
data/0CHOL_Arctica/Micrographs/*.mrc
data/0CHOL_Arctica/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 10% cholesterol
/data/10CHOL_Arctica
micrographs - single frame
MRC
MRC
653
1
32 BIT FLOAT
5760
1.14
4092
1.14
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
data/10CHOL_Arctica/Micrographs/*.mrc
data/10CHOL_Arctica/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 20% cholesterol
/data/20CHOL_Artica
micrographs - single frame
MRC
MRC
816
1
32 BIT FLOAT
5760
1.14
4092
1.14
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
data/20CHOL_Artica/Micrographs/*.mrc
data/20CHOL_Artica/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 30% cholesterol
/data/30CHOL_Artica
micrographs - single frame
MRC
MRC
492
1
32 BIT FLOAT
5760
1.14
4092
1.14
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
data/30CHOL_Artica/Micrographs/*.mrc
data/20CHOL_Artica/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 40% cholesterol
/data/40CHOL_Arctica
micrographs - single frame
MRC
MRC
481
1
32 BIT FLOAT
5760
0.715
4092
0.715
Micrographs were acquired at 0.1 s per frame with an 11.5 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
data/40CHOL_Arctica/Micrographs/*.mrc
data/40CHOL_Arctica/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with lipid mixture containing POPC instead of SDPC
/data/POPC_Arctica
micrographs - single frame
MRC
MRC
502
1
32 BIT FLOAT
11520
0.715
8184
0.715
Micrographs were acquired at 0.1 s per frame with an 11.5 s total exposure. Particle coordinates with refined alignments for the 34 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
data/POPC_Arctica/Micrographs/*.mrc
data/POPC_Arctica/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer without any brominated lipids
/data/NoBr_Krios
micrographs - single frame
MRC
MRC
5684
1
32 BIT FLOAT
5760
0.835
4092
0.835
Micrographs were acquired at 0.05 s per frame with an 5.9 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/NoBr_Krios/Micrographs/*.mrc
data/NoBr_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated cholesterol
/data/CHOL-Br_Krios
micrographs - single frame
MRC
MRC
1855
1
32 BIT FLOAT
5760
0.822
4092
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/CHOL-Br_Krios/Micrographs/*.mrc
data/CHOL-Br_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with iodinated cholesterol
/data/CHOL-I_Krios
micrographs - single frame
MRC
MRC
3186
1
32 BIT FLOAT
5760
0.822
4092
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/CHOL-I_Krios/Micrographs/*.mrc
data/CHOL-I_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated PIP2
/data/PIP2-Br_Krios
micrographs - single frame
MRC
MRC
2118
1
32 BIT FLOAT
5760
0.835
4092
0.835
Micrographs were acquired at 0.05 s per frame with an 5.9 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/PIP2-Br_Krios/Micrographs/*.mrc
data/PIP2-Br_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated POPS
/data/POPS-Br_Krios
micrographs - single frame
MRC
MRC
7806
1
32 BIT FLOAT
5760
0.822
4092
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/POPS-Br_Krios/Micrographs/*.mrc
data/POPS-Br_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated SDPC
/data/SDPC-Br_Krios
micrographs - single frame
MRC
MRC
2857
1
32 BIT FLOAT
5760
0.822
4096
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/SDPC-Br_Krios/Micrographs/*.mrc
data/SDPC-Br_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 25% brominated SDPC
/data/25SDPC-Br_Krios
micrographs - single frame
MRC
MRC
2931
1
32 BIT FLOAT
5760
0.822
4092
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/25SDPC-Br_Krios/Micrographs/*.mrc
data/25SDPC-Br_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 50% brominated SDPC
/data/50SDPC-Br_Krios
micrographs - single frame
MRC
MRC
3891
1
32 BIT FLOAT
5760
0.822
4092
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/50SDPC-Br_Krios/Micrographs/*.mrc
data/50SDPC-Br_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9A/F13A)/IST1 copolymer with brominated SDPC
/data/SDPC-Br_F9A_F13A_Krios
micrographs - single frame
MRC
MRC
1715
1
32 BIT FLOAT
5760
0.822
4092
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/SDPC-Br_F9A_F13A_Krios/Micrographs/*.mrc
data/SDPC-Br_F9A_F13A_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9E/F13E)/IST1 copolymer with brominated SDPC
/data/SDPC-Br_F9E_F13E_Krios
micrographs - single frame
MRC
MRC
1420
1
32 BIT FLOAT
5760
0.822
4092
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/SDPC-Br_F9E_F13E_Krios/Micrographs/*.mrc
data/SDPC-Br_F9E_F13E_Krios/*.star
Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9L/F13L)/IST1 copolymer with brominated SDPC
/data/SDPC-Br_F9L_F13L_Krios
micrographs - single frame
MRC
MRC
2188
1
32 BIT FLOAT
5760
0.822
4092
0.822
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
data/SDPC-Br_F9L_F13L_Krios/Micrographs/*.mrc
data/SDPC-Br_F9L_F13L_Krios/*.star