REL 2022-11-14 2022-12-05 2022-12-05 0000-0002-0799-3450 Martin Luke Rennie School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow University Ave Glasgow Lanarkshire United Kingdom G128QQ 0000-0002-4289-4810 Helen Walden School of Molecular Biosciences, College of Medical, Veterinary & Life Sciences, University of Glasgow University Ave Glasgow Lanarkshire United Kingdom G12 8QQ Rennie ML Walden H 1.6 10.6019/EMPIAR-11305 EMDB molecule EMD-11934 Rennie ML Arkinson C Chaugule VK Toth R Walden H Nature structural & molecular biology Nat Struct Mol Biol 4 28 356 364 2021 English 10.1038/s41594-021-00576-8 33795880

Raw movie frames without alignment, without gain correction. Accumulating 65 electrons per squared Angstrom over 59 frames for an exposure time of 14.9 seconds. relion_convert_to_tiff was used to undo gain correction and convert MRCs to LZW compressed TIFFs.

Unaligned multi-frame micrographs of reconstituted USP1, UAF1, FANCI, monoubiquitinated FANCD2, and DNA /data micrographs - multiframe TIFF TIFF 4593 59 32 BIT FLOAT 4096 1.015 4096 1.015

Multi-frame raw micrographs without gain correction in LZW compressed TIFF were generated from gain-corrected MRCs using relion_convert_to_tiff. Two batches of micrographs are uploaded (data/batch*/*.tif), each with a separate gain reference. Imaging was performed at 120,000x magnification on JEOL CryoARM 300 equipped with a DE64 detector at SCMI on 2020-08-17. Sample contained 8.8 μM of each of USP1(C90S), UAF1, FANCI, FANCD2 monoubiquitinated on K561 and dsDNA (61 base pairs).