REL 2022-10-21 2023-02-17 2023-02-17 0000-0002-7298-4841 Chloe Burnside The Rockefeller University New York United States 10065 0000-0002-9373-4737 Sebastian Klinge The Rockefeller University New York United States 10065 Harper NJ Burnside C Klinge S 73.6 10.6019/EMPIAR-11313 EMDB molecule EMD-26966 EMD-26967 EMD-26968 EMD-26969 EMD-26970 EMD-26971 EMD-27249 EMD-27250 EMD-27251 Harper NJ Burnside C Klinge S Nature Nature 7946 614 175 181 2022 English 10.1038/s41586-022-05621-0 36482135 Unaligned multi-frame micrographs of Ccm1-bound yeast mtSSU assembly intermediates /data/Krios2_11_11_21/tiffs micrographs - multiframe TIFF TIFF 31995 50 UNSIGNED BYTE 11520 0.54 8184 0.54

Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 31,995 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.

Unaligned multi-frame micrographs of Rsm22-bound yeast mtSSU assembly intermediates /data/Krios2_3_19_21/tiffs micrographs - multiframe TIFF TIFF 14111 40 UNSIGNED BYTE 11520 0.54 8184 0.54

Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 14,111 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.

Unaligned multi-frame micrographs of METTL17-bound human mtSSU assembly intermediates (dataset 1) /data/tifmerge micrographs - multiframe TIFF TIFF 37112 42 UNSIGNED BYTE 11520 0.54 8184 0.54

Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 37,112 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.

Unaligned multi-frame micrographs of METTL17-bound human mtSSU assembly intermediates (dataset 2) /data/3x3tiffs micrographs - multiframe TIFF TIFF 9990 42 UNSIGNED BYTE 11520 0.54 8184 0.54

Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 9,990 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.