REL 2022-12-03 2023-05-26 2023-10-09 Cryo-EM SPA datasets for Antibody 4-33 in complex with prefusion SARS-CoV-2 Spike glycoprotein (K3 movies/.tif files) 0000-0002-2910-0908 Ryan Gavin Casner Columbia University 3227 Broadway New York NY United States 10027 0000-0001-9943-8819 Lawrence Shapiro Department of Biochemistry, Columbia University 3227 Broadway New York NY United States 10027 Casner RG Reddem ER Shapiro L 1.8 10.6019/EMPIAR-11336 EMDB molecule EMD-26964 Liu L Casner RG Guo Y Wang Q Iketani S Chan JF Yu J Dadonaite B Nair MS Mohri H Reddem ER Yuan S Poon VK Chan CC Yuen KY Sheng Z Huang Y Bloom JD Shapiro L Ho DD Antibodies targeting a quaternary site on SARS-CoV-2 spike glycoprotein prevent viral receptor engagement by conformational locking Immunity Immunity 2023 English 10.1016/j.immuni.2023.09.003 37776849
Cryo-EM data for single particle analysis were collected at the Columbia Zuckerman Institute on a Titan Krios electron microscope operating at 300 kV, equipped with a Gatan K3-BioQuantum direct detection detector, using SerialEM. Exposures were taken at a magnification of 81,000x (pixel size of 1.07 Å), using a total electron fluence of 42 e-/Å2 fractionated over 60 frames with an exposure time of 3 s. A random defocus range of -0.8 to -2.0 µm was used.
 K3 Movies (.tif) for 4-33 Antibody + Spike /data/4-33/movies micrographs - multiframe TIFF TIFF 6976 60 UNSIGNED BYTE 5760 1.07 4092 1.07
Collected on the Columbia Zuckerman Institute Krios using K3-BioQuantum. Dose: 16 e/pix/s, Pixel Size: 1.074 A/pix, Exposure time 3s, Frame time 0.05s, Total Dose 42e/A2
data/4-33/movies/*.tif