REL 2023-01-04 2023-10-10 2023-10-10 SBF-SEM micrographs of A. algerae spores, Ungerminated 0000-0003-0624-6178 Gira Bhabha NYU School of Medicine New York City NY United States 10016 0000-0003-0624-6178 Gira Bhabha NYU School of Medicine New York City New York United States 10016 0000-0002-2570-0404 Damian Ekiert NYU School of Medicine New York City NY United States 10016 Jaroenlak P Cammer M Davydov A Sall J Usmani M Liang F Ekiert D Bhabha G 46.6 10.6019/EMPIAR-11369 SBF-SEM cell Jaroenlak P Cammer M Davydov A Sall J Usmani M Liang F Ekiert D Bhabha G 3-Dimensional organization and dynamics of the microsporidian polar tube invasion machinery PLoS pathogens PLoS Pathog United States 9 16 2020 English 10.1371/journal.ppat.1008738 32946515 SBF-SEM micrographs of A. algerae microsporidia spores, Ungerminated /data micrographs - single frame TIFF TIFF 250 1 UNSIGNED 32 BIT INTEGER 10000 2.2 10000 2.2
Micrographs imaged using the Gatan OnPoint BSE detector in a Zeiss Gemini 300 VP FESEM equipped with a Gatan 3View automatic microtome. The system was set to cut 50 nm slices, imaged with gas injection setting at 40% (2.9*10^-3 mBar) with Focus Charge Compensation to reduce electron accumulation charging artifacts. Images were recorded after each round of sectioning from the blockface using the SEM beam at 1.5 keV with a beam aperture size of 30 μm and a dwell time of 1.0 μsec/pixel.