REL 2023-01-06 2023-02-10 2023-02-10 A surface morphometrics toolkit to quantify organellar membrane ultrastructure using cryo-electron tomography 0000-0001-6346-5137 Michaela Medina Department of Integrative Structural and Computational Biology, The Scripps Research Institute 10550 N Torrey Pines Rd La Jolla California United States 92037 0000-0001-6346-5137 Michaela Medina Department of Integrative Structural and Computational Biology, The Scripps Research Institute 10550 N Torrey Pines Rd La Jolla California United States 92037 Barad BA Medina M Fuentes D Wiseman RL Grotjahn DA 126.9 10.6019/EMPIAR-11370 Exception cell Barad BA Medina M Fuentes D Wiseman RL Grotjahn DA A surface morphometrics toolkit to quantify organellar membrane ultrastructure using cryo-electron tomography bioRxiv 2022 10.1101/2022.01.23.477440 Unaligned tilt series of mouse embrionic fibroblasts with mitochondrial targeted GFP treated with vehicle or thapsigargin /data/Tilt_Series tilt series MRC MRC 34 10 UNSIGNED 16 BIT INTEGER 3710 variable 3838 variable
Single tilt axis tilt series. Tilt range is variable. For tilt series where frames were acquired the number of frames is 10 and the motioncorrected tilt series is given. Tilt series are from multiple data sets and have either a pixel spacing of 3.345 or 3.11, please refer to associated .mdoc file.
 Reconstructed tomograms used for segmentation of mitochondria and endoplasmic reticulum membranes /data/Tomograms reconstructed volumes MRC MRC 34 1 UNSIGNED 16 BIT INTEGER 960 variable 928 variable
Bin 4 reconstructed volumes from tilt series.
data/Voxel_Segmentations The voxel segmentations from AMIRA of membranes from mitochondria and ER in mouse embrionic fibroblasts treated with vehicle or thapsigargin REC data/Membrane_surface_mesh Polygon Surface meshes generated from voxel segmentations using 'Surface_Morphometrics' for all membranes from mitochondria and ER. STL