REL
2022-10-06
2023-04-12
2023-04-13
Cryo-Electron Microscopy structure of OmcZ nanowires from Geobacter sulfurreducens
0000-0002-8206-8804
Yangqi
Gu
Medical Research Council Laboratory of Molecular Biology
Cambridge Biomedical Campus, Francis Crick Ave
Cambridge
Cambridgeshire
United Kingdom
CB2 9FX
0000-0002-8206-8804
Yangqi
Gu
Medical Research Council Laboratory of Molecular Biology
Cambridge Biomedical Campus, Francis Crick Ave
Cambridge
Cambridgeshire
United States
06516
0000-0001-5611-6633
Nikhil
Malvankar
Yale University
840 West Campus Drive
West Haven
Connecticut
United States
06516
Gu YG
Srikanth V
Salazar-Morales AI
Samatey FA
Malvankar NS
Guberman-Pfeffer MGP
Shen CS
Gupta KG
Londer YL
Giska FG
Batista VB
National Science Foundation (NSF, China)
1749662
United States
National Science Foundation (NSF, China)
2210473
United States
National Science Foundation (NSF, China)
2038000
United States
National Institutes of Health/National Library of Medicine (NIH/NLM)
1DP2AI138259-01
United States
National Institutes of Health/National Library of Medicine (NIH/NLM)
R01GM141192
United States
Defense Advanced Research Projects Agency (DARPA)
W911NF-18-2-0100
United States
6.5
10.6019/EMPIAR-11439
EMDB
molecule
EMD-23481
Gu Y
Guberman-Pfeffer MJ
Srikanth V
Shen C
Giska F
Gupta K
Londer Y
Samatey FA
Batista VS
Malvankar NS
Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity
Nature microbiology
Nat Microbiol
United Kingdom
2
8
284
298
2023
English
10.1038/s41564-022-01315-5
36732469
Micrographs were recorded with super-resolution mode at 0.828 Å per pixel, with a total dose of ~60 e− Å−2 fractioned into 50 frames. A defocus range of 1–2.5 µm was used to collect ~14,500 images in total. A Quantum energy filter with a slit width at 20 eV was applied to remove inelastically scattered electrons.
Unaligned multi-frame micrographs using super resolution mode of OmcZ nanowire
/data/Micrographs
micrographs - multiframe
TIFF
TIFF
14005
50
1
50
UNSIGNED BYTE
11520
0.411
8184
0.411
Particles are autopicked helical particles generated by RELION 3.0.8. Micrographs are raw unaligned movies in super-resolution mode with a physical pixel size of 0.828 Å. Each micrograph contains 50 frames with a total dose of 60 e-/Å2. Super.mrc can be used as the gain reference for downstream motion correction.
data/Micrographs/OmcZ_micrographs_*/OMCZ_*.tif
data/Coordinates/OMCZ_*_autopick.star
data/Coordinates