REL 2023-03-07 2023-07-10 2023-07-10 0000-0002-4406-5151 Camilla Ventura Santos MRC Laboratory of Molecular Biology Cambridge Biomedical Campus, Francis Crick Ave Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0001-7292-5430 Andrew Carter MRC Laboratory of Molecular Biology Cambridge Biomedical Campus, Francis Crick Ave Cambridge Cambridgeshire United Kingdom CB2 0QH Ventura Santos C Carter AP Rogers SL Medical Research Council (MRC, United Kingdom) MC_UP_A025_1011 United Kingdom Welch Foundation 210711/Z/18/Z United Kingdom 491.9 10.6019/EMPIAR-11453 EMDB cell EMD-16800 EMD-16811 Ventura Santos C Carter AP Rogers SL Dataset 9 part 1: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset9_part1_221208/raw_movies micrographs - multiframe TIFF TIFF 28 14 UNSIGNED BYTE 5760 2.659 4092 2.659

Data from Dataset 9 part 1: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference is provided within the main folder. Data was collected on a K3 camera at a pixel size of 2.659 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder. TS_330 - TS_351 are from cofilin knock-down cells. TS_352 - TS_366 are from control knock-down cells.

Dataset 9 part 1: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset9_part1_221208/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 28 14 32 BIT FLOAT 920 11.81 1298 11.81

Dataset 9 part 1: Tomograms binned to 11.81 Å/ pixel, reconstructed and deconvolved in WARP. TS_330 - TS_351 are from cofilin knock-down cells. TS_352 - TS_366 are from control knock-down cells.

Dataset 9 part 2: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset9_part2_221217/raw_movies micrographs - multiframe TIFF TIFF 8 14 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 9 part 2: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder. TS_462 - TS_470 are from control knock-down cells.

Dataset 9 part 2: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset9_part2_221217/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 8 14 32 BIT FLOAT 960 11.81 928 11.81

Dataset 9 part 2: Tomograms binned by 4 (11.81 Å/ pixel), reconstructed and deconvolved in WARP. TS_462 - TS_470 are from control knock-down cells.

Dataset 10: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset10_221215/raw_movies micrographs - multiframe TIFF TIFF 34 14 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 10: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder. TS_424 - TS_442 are from cofilin knock-down cells. TS_443 - TS_461 are from control knock-down cells.

Dataset 10: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset10_221215/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 34 14 32 BIT FLOAT 960 11.81 928 11.81

Dataset 10: Tomograms binned by 4 (11.81 Å/ pixel), reconstructed and deconvolved in WARP. TS_424 - TS_442 are from cofilin knock-down cells. TS_443 - TS_461 are from control knock-down cells.

Dataset 11 part 1: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset11_230202_part1/raw_movies micrographs - multiframe TIFF TIFF 32 14 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 11 part 1: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder. TS_495 - TS_505 are from cofilin knock-down cells. TS_506 - TS_527 are from control knock-down cells.

Dataset 11 part 1: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset11_230202_part1/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 32 14 32 BIT FLOAT 960 11.8 928 11.8

Dataset 11 part 1: Tomograms binned by 4 (11.8 Å/ pixel), reconstructed and deconvolved in WARP. TS_495 - TS_505 are from cofilin knock-down cells. TS_506 - TS_527 are from control knock-down cells.

Dataset 11 part 2: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset11_221212_part2/raw_movies micrographs - multiframe TIFF TIFF 3 14 UNSIGNED BYTE 5760 2.659 4092 2.659

Data from Dataset 11 part 2: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference is provided within the main folder. Data was collected on a K3 camera at a pixel size of 2.659 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder. TS_367, TS_371, TS_380 are from cofilin knock-down cells.

Dataset 11 part 2: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset11_221212_part2/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 3 14 32 BIT FLOAT 1022 10.64 1440 10.64

Dataset 11 part 2: Tomograms binned by 4 (10.64 Å/ pixel), reconstructed and deconvolved in WARP. TS_367, TS_371, TS_380 are from cofilin knock-down cells.

Dataset 12: Raw multi-frame tilt images of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset12_230202/raw_movies micrographs - multiframe TIFF TIFF 27 14 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 12: Raw multi-frame movies of tilt series collected on protrusions of control or cofilin knock-down Drosophila S2 cells treated with 2.5 uM Cytochalsin D for 2 hours and 2 uM thapsigargin for 5 hours. The gain reference and defects files are provided within the main folder. Data was collected on a K2 summit camera at a pixel size of 2.952 Å / pixel. Metadata from tilt series acquisition (Mdoc files) is provided inside the Mdoc_files folder. TS_528 - TS_540 are from cofilin knock-down cells. TS_542 - TS_556 are from control knock-down cells.

Dataset 12: Binned, deconvolved tomograms of induced protrusions of control and cofilin knock-down Drosophila S2 cells /data/Dataset12_230202/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 27 14 32 BIT FLOAT 960 11.8 928 11.8

Dataset 12: Tomograms binned by 4 (11.8 Å/ pixel), reconstructed and deconvolved in WARP. TS_528 - TS_540 are from cofilin knock-down cells. TS_542 - TS_556 are from control knock-down cells.