REL 2022-11-16 2023-05-09 2023-05-11 Cryo-EM SPA datasets of broadly neutralizing antibody 2-36 in complex with prefusion SARS-CoV-2 and SARS-CoV spike glycoproteins (K3 movies/.tif files) 0000-0002-2910-0908 Ryan Gavin Casner Columbia University 3227 Broadway New York NY United States 10027 0000-0001-9943-8819 Lawrence Shapiro Department of Biochemistry, Columbia University 3227 Broadway New York NY United States 10027 Casner RG Cerutti G Shapiro L 2.5 10.6019/EMPIAR-11513 EMDB molecule EMD-24190 Wang P Casner RG Nair MS Yu J Guo Y Wang M Chan JF Cerutti G Iketani S Liu L Sheng Z Chen Z Yuen KY Kwong PD Huang Y Shapiro L Ho DD A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses Emerging microbes & infections Emerg Microbes Infect 1 11 147 157 2022 English 10.1080/22221751.2021.2011623 34836485
Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.
 K3 Movies (.tif) for 2-36 Antibody + SARS-CoV-2 Spike /data/SARS-CoV-2-Spike-dataset-movies micrographs - multiframe TIFF TIFF 4135 60 UNSIGNED BYTE 5760 1.07 4092 1.07
Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.
data/SARS-CoV-2-Spike-dataset-movies/20aug25a/*.tif K3 Movies (.tif) for 2-36 Antibody + SARS-CoV Spike /data/SARS-CoV-Spike-dataset-movies micrographs - multiframe TIFF TIFF 4980 60 UNSIGNED BYTE 5760 1.07 4092 1.07
Cryo-EM data for single particle analysis was collected on a Titan Krios electron microscope operating at 300 kV, equipped with an energy filter and a Gatan K3-BioQuantum direct detection detector, using the Leginon software package. Exposures were taken with a total electron fluence of 41.92 e-/Å2 fractionated over 60 frames, with a total exposure time of 3 s. A defocus range of −0.8 to −2.5 µm was used with a magnification of 81,000x, and a pixel size of 1.07 Å.
data/SARS-CoV-Spike-dataset-movies/20oct30a/*.tif