<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11556" schemaVersion="0.65" public="true">
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        <keyDates>
            <depositionDate>2023-05-23</depositionDate>
            <releaseDate>2024-07-19</releaseDate>
            <updateDate>2024-07-19</updateDate>
        </keyDates>
        <title>Single particle reconstruction of the human LINE-1 ORF2p without substrate (apo)</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-0549-1702</authorORCID>
            <firstName>Trevor</firstName>
            <middleName>Peter</middleName>
            <lastName>van Eeuwen</lastName>
            <organization type="academic">Laboratory of Cellular and Structural Biology</organization>
            <street>1230 York Ave</street>
            <townOrCity>New York</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>10065</postOrZipCode>
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        <principalInvestigator private="true">
            <authorORCID>0000-0003-0549-1702</authorORCID>
            <firstName>Michael</firstName>
            <middleName>P</middleName>
            <lastName>Rout</lastName>
            <organization type="academic">Laboratory of Cellular and Structural Biology</organization>
            <street>1230 York Ave</street>
            <townOrCity>New York</townOrCity>
            <stateOrProvince>NY</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>10065</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-0549-1702">van Eeuwen T</author>
            <author authorORCID="0000-0001-6987-8119">Zalevsky A</author>
            <author authorORCID="0000-0003-2010-706X">Rout MP</author>
            <author authorORCID="0000-0001-5824-142X">Taylor MS</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</fundingBody>
                <code>P41 GM109824</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.9</datasetSize>
        <entryDOI>10.6019/EMPIAR-11556</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-40856</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Baldwin ET</author>
                    <author authorORCID="0000-0003-0549-1702" order="2">van Eeuwen T</author>
                    <author order="3">Hoyos D</author>
                    <author authorORCID="0000-0001-6987-8119" order="4">Zalevsky A</author>
                    <author order="5">Tchesnokov EP</author>
                    <author order="6">Sánchez R</author>
                    <author authorORCID="0009-0006-9145-8499" order="7">Miller BD</author>
                    <author order="8">Di Stefano LH</author>
                    <author authorORCID="0000-0002-0457-0030" order="9">Ruiz FX</author>
                    <author order="10">Hancock M</author>
                    <author order="11">Işik E</author>
                    <author authorORCID="0000-0002-8190-8568" order="12">Mendez-Dorantes C</author>
                    <author order="13">Walpole T</author>
                    <author order="14">Nichols C</author>
                    <author order="15">Wan P</author>
                    <author order="16">Riento K</author>
                    <author order="17">Halls-Kass R</author>
                    <author order="18">Augustin M</author>
                    <author order="19">Lammens A</author>
                    <author order="20">Jestel A</author>
                    <author order="21">Upla P</author>
                    <author order="22">Xibinaku K</author>
                    <author authorORCID="0009-0001-5277-2235" order="23">Congreve S</author>
                    <author authorORCID="0009-0000-1589-1780" order="24">Hennink M</author>
                    <author authorORCID="0000-0001-5997-7770" order="25">Rogala KB</author>
                    <author order="26">Schneider AM</author>
                    <author authorORCID="0000-0002-2319-1773" order="27">Fairman JE</author>
                    <author order="28">Christensen SM</author>
                    <author order="29">Desrosiers B</author>
                    <author order="30">Bisacchi GS</author>
                    <author order="31">Saunders OL</author>
                    <author order="32">Hafeez N</author>
                    <author order="33">Miao W</author>
                    <author order="34">Kapeller R</author>
                    <author order="35">Zaller DM</author>
                    <author authorORCID="0000-0003-0435-6197" order="36">Sali A</author>
                    <author authorORCID="0000-0001-5818-6248" order="37">Weichenrieder O</author>
                    <author authorORCID="0000-0003-1620-3761" order="38">Burns KH</author>
                    <author authorORCID="0000-0001-6213-5806" order="39">Götte M</author>
                    <author authorORCID="0000-0003-2010-706X" order="40">Rout MP</author>
                    <author authorORCID="0000-0003-2612-9622" order="41">Arnold E</author>
                    <author authorORCID="0000-0001-6153-8793" order="42">Greenbaum BD</author>
                    <author authorORCID="0000-0002-3009-1965" order="43">Romero DL</author>
                    <author authorORCID="0000-0002-6307-7713" order="44">LaCava J</author>
                    <author authorORCID="0000-0003-1560-9276" order="45">Taylor MS</author>
                    <title>Structures, functions and adaptations of the human LINE-1 ORF2 protein</title>
                    <journal>Nature</journal>
                    <journalAbbreviation>Nature</journalAbbreviation>
                    <country></country>
                    <issue>7997</issue>
                    <volume>626</volume>
                    <firstPage>194</firstPage>
                    <lastPage>206</lastPage>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41586-023-06947-z</externalReferences>
                    <externalReferences type="pubmed">38096902</externalReferences>
                </journalCitation>
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        </citationList>
    </crossReferences>
    <imageSet>
        <name>Aligned single frame micrographs of apo LINE-1 ORF2p</name>
        <directory>/data/alignedMRC</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>6477</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.65</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.65</pixelHeight>
        </dimensions>
        <details>No associated particles</details>
        <segmentationList/>
        <micrographsFilePattern>data/alignedMRC/ApoL1*_ali.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multiframe movies of apo LINE-1 ORF2p</name>
        <directory>/data/rawTIFF</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6477</numImagesOrTiltSeries>
        <framesPerImage>38</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>38</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.325</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.325</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
