REL 2023-07-05 2023-08-18 2023-08-18 0000-0003-1136-6000 Richard Wayne Baker University of North Carolina at Chapel Hill 3049A Genetic Medicine Bldg Chapel Hill NC United States 27599 0000-0003-1136-6000 Richard Wayne Baker University of North Carolina at Chapel Hill 3049A Genetic Medicine Bldg Chapel Hill NC United States 27599 Partlow EA Cannon KS Hollopeter G Baker RW National Institutes of Health (NIH) R01GM127548 United States 267.5 10.6019/EMPIAR-11604 EMDB molecule EMD-24710 EMD-24711 Partlow EA Cannon KS Hollopeter G Baker RW Nature structural & molecular biology Nat Struct Mol Biol 4 29 339 347 2022 English 10.1038/s41594-022-00749-z 35347313

The micrographs are motion corrected and dose-weighted in cryoSPARC v3. The input movies were dose-fractionated stacks collected on a Talos Arctica with a K3 DED. The movies originally contained 60 frames, with a pixel size of 0.89 Angstrom, and a total dose of 55 e-/A^2. These micrographs were used to determine a structure of AP2 in the presence of heparin.

Motion corrected, dose-weighted micrographs from two data collection sessions /data micrographs - single frame MRC MRC 3046 1 32 BIT FLOAT 5760 0.89 4092 0.89

This is a dataset of the AP2 clathrin adaptor in the presence of heparin. Included are aligned and dose weighted micrographs from cryoSPARC v3. Two data collections were performed one week apart with identical microscope parameters. The data are in two separate directories split by collection date. Total micrographs: 3046 Pixel Size: 0.89 Ang Magnification = 45,000