2 datasets were collected.

Dataset1: 

550 movies (50 frames) collected as gain-normalised MRC files and converted to tiff with relion_convert_to_tiff. Use gain-reference.mrc as provided for gain reference. 

Calibrated pixel size: 1.07 A
Flux: 7.58 e-/pixel/s
Total fluence 53 e-/A^2

Dataset2: 

1874 movies (50 frames) collected as gain unnormalised MRC files and converted to tiff with relion_convert_to_tiff. Use gain.mrc as provided for gain reference. 

Calibrated pixel size: 1.07 A
Flux 7.65 e-/pixel/s
Total fluence 54 e-/A^2

consensus_refinement_data.star contains metadata for 174040 particles from Refine3D before focused classification with partial signal subtraction (2.92 A)
after_focused_classification_data.star contains metadata for 76626 particles from best class after focused classification with partial signal subtraction (3.0 A)