REL 2023-09-06 2023-10-17 2023-10-17 3D reconstructions of parasite development and the intracellular niche of the microsporidian pathogen E. intestinalis 0000-0003-0624-6178 Gira Bhabha Department of Cell Biology, NYU Grossman School of Medicine 430 E 29th Street New York New York United States 10016 0000-0003-0624-6178 Gira Bhabha Department of Cell Biology, NYU Grossman School of Medicine New York New York United States 10016 0000-0002-2570-0404 Damian Ekiert Department of Microbiology, NYU Grossman School of Medicine 430 E 29th Street New York New York United States 10016 Antao NVA Lam CKL Davydov AD Riggi MR Sall JS Petzold CP Liang FL Iwasa JI Ekiert DCE Bhabha GB 537.9 10.6019/EMPIAR-11683 SBF-SEM cell Riggi MR Antao NV Lam C Davydov A Sall J Petzold C Liang F Iwasa J Ekiert DC Bhabha G 3D reconstructions of parasite development and the intracellular niche of the microsporidian pathogen<i>E. intestinalis</i> bioRxiv United States 2023 English 10.1101/2023.07.02.547383 37425741
The micrographs have been deposited without any alignment or removal of skipped slices for 1)Vero cells infected with E.intestinalis for 24-h and 48-h, 2) Purified spores and 3) Uninfected cells
 24h infected cell 1 /data/24h_infected_cell_1 micrographs - single frame DM4 DM4 86 1 UNSIGNED 16 BIT INTEGER 20000 20.0 35000 20.0
Unaligned and unprocessed micrograph stack of a Vero cell at 24 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 24h infected cell 2 /data/24h_infected_cell_2 micrographs - single frame DM4 DM4 116 1 UNSIGNED 16 BIT INTEGER 30000 20.0 25000 20.0
Unaligned and unprocessed micrograph stack of a Vero cell at 24 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 48h infected cell 1 /data/48h_infected_cell_1 micrographs - single frame DM4 DM4 200 1 UNSIGNED 16 BIT INTEGER 12000 20.0 12000 20.0
Unaligned and unprocessed micrograph stack of a Vero cell at 48 hours post infection with E. intestinalis. Slice thickness=30nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 48h infected cell 2 /data/48h_infected_cell_2 micrographs - single frame DM4 DM4 83 1 UNSIGNED 16 BIT INTEGER 22000 20.0 23000 20.0
Unaligned and unprocessed micrograph stack of a Vero cell at 48 hours post infection with E. intestinalis. There are two ROIs associated with this infected cell: ROI00 (22000x23000) and ROI01 (22000x23000). ROI00 and ROI01 were stitched together for segmentation analysis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 48h infected cell 3 /data/48h_infected_cell_3 micrographs - single frame DM4 DM4 79 1 UNSIGNED 16 BIT INTEGER 25000 20.0 20000 20.0
Unaligned and unprocessed micrograph stack of a parasitophorous vacuole in a Vero cell at 48 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 48h infected cell 4 /data/48h_infected_cell_4 micrographs - single frame DM4 DM4 74 1 UNSIGNED 16 BIT INTEGER 14000 20.0 15000 20.0
Unaligned and unprocessed micrograph stack of a parasitophorous vacuole in a Vero cell at 48 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 48h infected cell 5 /data/48h_infected_cell_5 micrographs - single frame DM4 DM4 50 1 UNSIGNED 16 BIT INTEGER 14000 20.0 15000 20.0
Unaligned and unprocessed micrograph stack of a parasitophorous vacuole in a Vero cell at 48 hours post infection with E. intestinalis. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 Purified spores dataset 1 /data/Purified_spores_1 micrographs - single frame DM4 DM4 140 1 UNSIGNED 16 BIT INTEGER 5000 20.0 5000 20.0
E. intestinalis spores purified from Vero cells. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 Purified spores dataset 2 /data/Purified_spores_2 micrographs - single frame DM4 DM4 140 1 UNSIGNED 16 BIT INTEGER 5000 20.0 5000 20.0
E. intestinalis spores purified from Vero cells. Slice thickness=50nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 Uninfected cell 1 /data/Uninfected_cell_1 micrographs - single frame DM4 DM4 50 1 UNSIGNED 16 BIT INTEGER 12500 25.0 12500 25.0
Partial volume of an uninfected Vero cell. Slice thickness=60nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.
 Uninfected cell 2 /data/Uninfected_cell_2 micrographs - single frame DM4 DM4 50 1 UNSIGNED 16 BIT INTEGER 12500 25.0 12500 25.0
Partial volume of an uninfected Vero cell. Slice thickness=60nm. Images were processed in Fiji using the Gaussian blur filter with a sigma (radius=2) prior to analysis in Dragonfly.