<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11723" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2023-10-11</depositionDate>
            <releaseDate>2024-11-08</releaseDate>
            <updateDate>2024-11-08</updateDate>
        </keyDates>
        <title>Cryo-EM structure of the gastric proton pump</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-2681-5921</authorORCID>
            <firstName>Kazuhiro</firstName>
            <lastName>Abe</lastName>
            <organization type="academic">Cellular and Structural Physiology Institute, Nagoya University</organization>
            <street>1, Furo-cho, Chikusa-ku</street>
            <townOrCity>Nagoya</townOrCity>
            <stateOrProvince>Aichi</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>464-8601</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-2681-5921</authorORCID>
            <firstName>Kazuhiro</firstName>
            <lastName>Abe</lastName>
            <organization type="academic">Cellular and Structural Physiology Institute, Nagoya University</organization>
            <street>1, Furo-cho, Chikusa-ku</street>
            <townOrCity>Nagoya</townOrCity>
            <stateOrProvince>Aichi</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>464-8601</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-2681-5921">Abe K</author>
            <author>Yokoshima S</author>
            <author>Yoshimori A</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">7.3</datasetSize>
        <entryDOI>10.6019/EMPIAR-11723</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-35500</emdbEntry>
            <emdbEntry>EMD-35501</emdbEntry>
            <emdbEntry>EMD-35502</emdbEntry>
            <emdbEntry>EMD-36424</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Abe K</author>
                    <author order="2">Ozako M</author>
                    <author order="3">Inukai M</author>
                    <author order="4">Matsuyuki Y</author>
                    <author order="5">Kitayama S</author>
                    <author order="6">Kanai C</author>
                    <author order="7">Nagai C</author>
                    <author order="8">Gopalasingam CC</author>
                    <author order="9">Gerle C</author>
                    <author order="10">Shigematsu H</author>
                    <author order="11">Umekubo N</author>
                    <author order="12">Yokoshima S</author>
                    <author order="13">Yoshimori A</author>
                    <title>Deep learning driven de novo drug design based on gastric proton pump structures.</title>
                    <journal>Communications Biology</journal>
                    <journalAbbreviation>Commun Biol</journalAbbreviation>
                    <country></country>
                    <volume>6</volume>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s42003-023-05334-8</externalReferences>
                    <externalReferences type="pubmed">37726448</externalReferences>
                    <details>gastric proton pump with de novo designed drug.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>K3 movies for gastric proton pump with DQ-02</name>
        <directory>/data/8IJV/</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>9650</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>60</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.753</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.753</pixelHeight>
        </dimensions>
        <details>- Please use 'CountCDSRef_2022-11-24_14.24.17_220830B06_51-1_0000.mrc' as a gain reference without rotation or flipping in RELION.
- The flux is 1 e/A2/frame.
- Cs is 2.7 mm.
- Four digits in the file name represent image shift directions.
- Movies were recorded with 5x5 image shift with two shots per hole.
- The pixel size in the image header is not correct.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>K3 movies for gastric proton pump with DQ-06</name>
        <directory>/data/8IJW/</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>8100</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>60</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.58</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.58</pixelHeight>
        </dimensions>
        <details>- Please use 
  'CountCDSRef_2022-10-21_12.30.19_220830C12_76-3_0000.mrc' for 'Movies' and 
  'CountCDSRef_2022-10-21_22.12.15_220830C12_82-17_0000.mrc' for 'Movies2' 
as a gain reference without rotation or flipping in RELION.
- The flux is 1 e/A2/frame.
- Cs is 2.7 mm.
- Four digits in the file name represent image shift directions.
- Movies were recorded with 5x5 image shift with two shots per hole.
- The pixel size in the image header is not correct.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>K3 movies for gastric proton pump with DQ-18</name>
        <directory>/data/8IJX/</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>9200</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>60</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.753</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.753</pixelHeight>
        </dimensions>
        <details>- Please use 'CountCDSRef_2022-12-20_13.53.39_221213A02_67-1_0000.mrc' as a gain reference without rotation or flipping in RELION.
- The flux is 1 e/A2/frame.
- Cs is 2.7 mm.
- Four digits in the file name represent image shift directions.
- Movies were recorded with 5x5 image shift with two shots per hole.
- The pixel size in the image header is not correct.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>K3 movies for gastric proton pump with DQ-21</name>
        <directory>/data/8JMN/</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7844</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.753</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.753</pixelHeight>
        </dimensions>
        <details>- Please use 'CountCDSRef_2023-04-27_15.38.01_230418C11_66-1_0000.mrc' as a gain reference without rotation or flipping in RELION.
- The flux is 1 e/A2/frame.
- Cs is 2.7 mm.
- Four digits in the file name represent image shift directions.
- Movies were recorded with 5x5 image shift with two shots per hole.
- The pixel size in the image header is not correct.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
