REL 2023-10-20 2024-10-08 2024-10-08 0000-0003-4247-4303 Ricardo Diogo Righetto Biozentrum, University of Basel Basel Switzerland 4056 0000-0002-0941-4387 Benjamin D. Engel Biozentrum, University of Basel Basel Switzerland 4056 Demulder M Righetto RD Wietrzynski W Lamm L Engel BD European Research Council (ERC) M822.00045 Switzerland 81.8 10.6019/EMPIAR-11747 EMDB cell EMD-18709 Shimakawa G Demulder M Flori S Kawamoto A Tsuji Y Nawaly H Tanaka A Tohda R Ota T Matsui H Morishima N Okubo R Wietrzynski W Lamm L Righetto RD Uwizeye C Gallet B Jouneau PH Gerle C Kurisu G Finazzi G Engel BD Matsuda Y 10.1016/j.cell.2024.09.013 Denoised bin4 tomograms /data/bin4_cryocare reconstructed volumes MRC MRC 7 512 32 BIT FLOAT 1024 8.572 1440 8.572

Tomograms denoised using cryo-CARE trained on each individual tomogram reconstructed from odd/even frames.

IMOD bin4 tomograms /data/bin4_imod reconstructed volumes MRC MRC 7 512 32 BIT FLOAT 1024 8.572 1440 8.572

IMOD reconstruction at bin4 using weighted backprojection without CTF correction. IMOD reconstruction files (.xf and .tlt) are provided in the imod_files/ directory.

Tilt series assembled from motion-corrected and averaged frames /data/raw_stacks tilt series MRC MRC 7 53 32 BIT FLOAT 5760 2.143 4096 2.143

Frames aligned with MotionCor2 Note: Frames per image varies

Raw frames /data/raw_frames micrographs - multiframe TIFF TIFF 327 5 UNSIGNED BYTE 11520 1.071 8184 1.071

Movies acquired with a K3 detector in super-resolution mode using SerialEM. Gain reference and defects file are provided in the gainref/ directory. Metadata from the acquisition can be found in the mdocs/ directory.