REL 2024-01-09 2025-02-24 2025-02-24 Cryo-ET of Dictyostelium discoideum in 0.4 M sorbitol (hyperosmotic stress) 0000-0003-3421-6363 Patrick C Hoffmann MPI of Biophysics Frankfurt Germany 60438 0000-0002-7397-1321 Martin Beck MPI of Biophysics Frankfurt Germany 60438 Hoffmann PC Beck M 332.1 10.6019/EMPIAR-11943 EMDB organism EMD-19155 Hoffmann PC Kim H Obarska-Kosinska A Kreysing JP Andino-Frydman E Cruz-Leon S Cernikova L Kosinski J Turonova B Hummer G Beck M Nuclear pores as conduits for fluid flow during osmotic stress Raw tilt series of D. dicoideum cells (hyperosmotic stress condition) - frame aligned /data tilt series MRC MRC 59 10 SIGNED 16 BIT INTEGER variable variable variable variable
Tilt series were frame-aligned in SerialEM during acquisition, aligned mrc and mdoc files are provided, TS overview files contain information about acquisition parameters and excluded tilt series images from raw stacks, alignment files (.xf) and tilt files (.tlt) were generated from IMOD (note that some tilt images may be excluded, for more information see TiltSeries_Info_hypertonic.xlsx file), CTF estimation from gctf are included as gctf.star files.